ELISA Kit DIY Materials for Prolactin (PRL)
LTH; Luteotropic Hormone
- UOM
- FOB US$ 1,512.00 US$ 2,520.00 US$ 4,536.00 US$ 8,820.00 US$ 15,120.00
- Quantity
Overview
Properties
- Product No.KSA846Bo01
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only - Downloadn/a
- CategoryEndocrinologyReproductive scienceGenetic scienceHormone metabolism
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range1.56-100ng/mL
- Applicable Sensitivity0.64ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Prolactin (PRL). No significant cross-reactivity or interference between Prolactin (PRL) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- In Vivo Evidence for Epidermal Growth Factor Receptor (EGFR)-mediated Release of Prolactin from the Pituitary GlandJcb: 39297
- TLR9 expression is associated with prognosis in patients with glioblastoma multiformeScienceDirect: S0967586811003572
- Determination of prolactin hormone in serum and urine using an electrochemical immunosensor based on poly(pyrrolepropionic acid)/carbon nanotubes hybrid modified electrodesSciencedirect:S0925400514000720
- Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifersPubmed:27051212
- Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calvespublication:306322503
- Differentiation of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells into endometrial cellspubmed:29096715
- The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicityPubmed:30021657
- Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in MicePubmed:29403355
- The antipsychotics sulpiride induces fatty liver in rats via phosphorylation of insulin receptor substrate-1 at Serine 307-mediated adipose tissue insulin resistancePubmed:29551354
- The prolactin‐release inhibitor paeoniflorin suppresses proliferation and induces apoptosis in prolactinoma cells via the mitochondria‐dependent pathwayPubmed:29388711
- Metformin inhibits growth and prolactin secretion of pituitary prolactinoma cells and xenograftsPubmed: 30334324
- Hormonal and metabolic indicators before and after farrowing in sows affected with postpartum dysgalactia syndromePubmed: 30404636
- Expression of prolactin receptors in the duodenum, kidneys and skeletal system during physiological and sulpiride-induced hyperprolactinaemiaPubmed: 30143940
- Morphological and Biochemical Characteristics of Prostate Hyperplasia during Sulpiride TreatmentPubmed: 32152847
- Effect of heat stress during the early and late dry period on mammary gland development of Holstein dairy cattlePubmed: 32684470
- Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat StressPubmed: 32685488
- Prolactin affects the disappearance of ALV-J viremia in vivo and inhibits viral infection34391195
- Effects of a Nanoencapsulated Moringa Leaf Ethanolic Extract on the Physiology, Metabolism and Reproductive Performance of Rabbit Does during Summer34439574
- Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome MicePubmed:35057459