ELISA Kit DIY Materials for Cluster of Differentiation 59 (CD59)
Protectin; MIC11; MIN1; MIN2; MIN3; MSK21; MAC-IP; MACIF; MIRL; HRF20; Complement Regulatory Protein; 20 kDa homologous restriction factor; Membrane attack complex inhibition factor
- UOM
- FOB US$ 1,190.00 US$ 1,984.00 US$ 3,571.00 US$ 6,944.00 US$ 11,904.00
- Quantity
Overview
Properties
- Product No.KSB336Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only - Downloadn/a
- CategoryCD & Adhesion moleculeTumor immunityHematology
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, urine and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range62.50-4000pg/mL
- Applicable Sensitivity25.5pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Protectin (CD59). No significant cross-reactivity or interference between Protectin (CD59) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Soluble CD59 is a Novel Biomarker for the Prediction of Obstructive Chronic Lung Allograft Dysfunction After Lung TransplantationPubmed:27215188
- Salivary levels of last generation specific pro©\resolving lipid mediators (SPMs)(protectin and maresin) in patients with cardiovascular and periodontal disease: A case ¡33650687