ELISA Kit DIY Materials for Prothrombin Fragment 1+2 (F1+2) Mus musculus (Mouse) Assay Kit DIY Materials

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.312-20ng/mL
  • Applicable Sensitivity0.114ng/mL
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  • ELISA Kit DIY Materials for Prothrombin Fragment 1+2 (F1+2) Packages (Simulation)
  • ELISA Kit DIY Materials for Prothrombin Fragment 1+2 (F1+2) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Prothrombin Fragment 1+2 (F1+2). No significant cross-reactivity or interference between Prothrombin Fragment 1+2 (F1+2) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Disseminated intravascular coagulation or acute coagulopathy of trauma shock early after trauma? An observational studyBioMed: cc10553
  • Changes in thrombin generation, fibrinolysis, platelet and endothelial cell activity, and inflammation following endovascular abdominal aortic aneurysm repairScienceDirect: S0741521411018568
  • High levels of soluble VEGF receptor 1 early after trauma are associated with shock, sympathoadrenal activation, glycocalyx degradation and inflammation in severely injured patients: a prospective studySjtrem:1757-7241
  • High sCD40L levels early after trauma are associated with enhanced shock, sympathoadrenal activation, tissue and endothelial damage, coagulopathy and mortalityWiley: source
  • Evaluation of autologous plasma skin test in patients with chronic idiopathic urticariaPubMed: 21910697
  • Diet Modulates Endogenous Thrombin Generation, A Biological Estimate of Thrombosis Risk, Independently of the Metabolic StatusPubMed: 22859493
  • Medium-term effect of endovascular and open abdominal aortic aneurysm repair on thrombin generation and fibrinolysisPubMed: 23140799
  • Markers of Thrombogenesis and Fibrinolysis and Their Relation to Inflammation and Endothelial Activation in Patients with Idiopathic Pulmonary Arterial HypertensionPlosone: Source
  • Effect of endovascular and open abdominal aortic aneurysm repair on thrombin generation and fibrinolysisPubmed: 23140799
  • Clinical impact of factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations among sickle cell disease patients of Central IndiaPubmed: 23992124
  • Study the association of adiponectin with inflammation and hypercoagulability in case of type 2 diabetic subjects with renal dysfunctionTandfonline:Source
  • An ex vivo evaluation of blood coagulation and thromboresistance of two extracorporeal circuit coatings with reduced and full heparin dosePubmed:24632424
  • Ex vivo simulation of cardiopulmonary bypass with human blood for hemocompatibility testingPubMed: 26243277
  • Comparison of the Effect of Dabigatran and Dalteparin on Thrombus Stability in a Murine Model of Venous ThromboembolismPubMed: 26514101
  • Thrombin activatable fibrinolysis inhibitor (TAFI), tissue factor pathway inhibitor (TFPI), and prothrombin fragment 1+2 levels in patients with advanced colorectal cancer aot:AOT_49_1_6_12.pdf
  • Perioperative thrombocytopenia predicts poor outcome in patients undergoing transcatheter aortic valve implantationpubmed:29145170
  • The causes of thrombocytopenia after transcatheter aortic valve implantationpubmed:28582640
  • Elevations of Thrombotic Biomarkers in Hemoglobin H DiseasePubmed:29402840
  • The cerebral thrombin system is activated after intracerebral hemorrhage and contributes to secondary lesion growth and poor neurological outcome in C57Bl/6 micePubmed: 31830857
  • Enhanced Fibrin-Lysis in Grade-1 Dengue Haemorrhagic Fever
  • The impact of anti-endothelial cell antibodies (AECAs) on the development of blood vessel damage in patients with systemic lupus erythematosus: the preliminary …Pubmed:35284968

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