ELISA Kit DIY Materials for S100 Calcium Binding Protein A7 (S100A7) Bos taurus; Bovine (Cattle) Assay Kit DIY Materials

S100-A7; PSOR1; S100A7c; Psoriasin 1

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluid.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.070ng/mL
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  • ELISA Kit DIY Materials for S100 Calcium Binding Protein A7 (S100A7) Packages (Simulation)
  • ELISA Kit DIY Materials for S100 Calcium Binding Protein A7 (S100A7) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein A7 (S100A7). No significant cross-reactivity or interference between S100 Calcium Binding Protein A7 (S100A7) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Serum levels of antimicrobial peptides and proteins do not correlate with psoriasis severity and are increased after treatment with fumaric acid estersSpringerLink: t07u24t43r357l15
  • Proteomic Signature of Endothelial Dysfunction Identified in the Serum of Acute Ischemic Stroke Patients by the iTRAQ-Based LC–MS ApproachPubMed: 25807139
  • S100A7 (psoriasin) inhibits human epidermal differentiation by enhanced IL-6 secretion through IκB/NF-κB signalingPubmed:27060579
  • S100A7 (psoriasin) inhibits human epidermal differentiation by enhanced IL‐6 secretion through IκB/NF‐κB signallingpubmed:27060579
  • Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescriptionpubmed:28672924
  • The psoriasis-associated IL-17A induces and cooperates with IL-36 cytokines to control keratinocyte differentiation and function.pubmed:29142248
  • A potential contribution of psoriasin to vascular and epithelial abnormalities and inflammation in systemic sclerosisPubmed:28681537
  • Measurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping techniquePubmed:29374283
  • Comprehensive proteomic profiling of patients' tears identifies potential biomarkers for the traumatic vegetative statePubmed:30019218
  • Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detectionPubmed: 31706945
  • Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris

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