ELISA Kit DIY Materials for Salusin Beta (SALb) Rattus norvegicus (Rat) Assay Kit DIY Materials

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range4.94-400pg/mL
  • Applicable Sensitivity1.92pg/mL
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  • ELISA Kit DIY Materials for Salusin Beta (SALb) Packages (Simulation)
  • ELISA Kit DIY Materials for Salusin Beta (SALb) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Salusin Beta (SALb). No significant cross-reactivity or interference between Salusin Beta (SALb) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Serum levels of apelin, salusin-alpha, and salusin-beta in normal pregnancy and preeclampsiaInforma: 14767058.2012.660221
  • The bioactive peptides salusins and apelin–36 are produced in human arterial and venous tissues and the changes of their levels during cardiopulmonary bypassPubMed: 22884920
  • Presence of adropin, nesfatin-1, apelin-12, ghrelins and salusins peptides in the milk, cheese whey and plasma of dairy cowsScienceDirect: S0196978113000533
  • Association of low maternal levels of salusins with gestational diabetes mellitus and with small-for-gestational-age fetuses.PubMed: 23178004
  • Serum concentration and kidney expression of salusin-α and salusin-β in rats with metabolic syndrome induced by fructose.PubMed: 23244237
  • Salusins, newly identified regulators of hemodynamics and mitogenesis, increase in polycystic ovarian syndrome.PubMed: 22827297
  • Concentrations of preptin, salusins and hepcidins in plasma and milk of lactating women with or without gestational diabetes mellitusScienceDirect: S0196978113003100
  • Salusin-alpha and -beta expression in heart and aorta with and without metabolic syndrome Com:Source
  • Brain, Liver, and Serum Salusin-alpha and-beta Alterations in Sprague-Dawley Rats with or Without Metabolic SyndromePubmed:Pmc4136931
  • Superoxide anions involved in sympathoexcitation and pressor effects of salusin-β in paraventricular nucleus in hypertensive ratsPubmed:24304512
  • Is the serum level of salusin-β associated with hypertension and atherosclerosis in the pediatric population?Pubmed:25245503
  • Serum salusin-α and salusin-β levels in patients with psoriasisPubMed: 26055619
  • Serum Salusin-β Levels Are Associated With the Presence and Severity of Coronary Artery DiseasePubMed: 25730454
  • Serum Salusin-β Levels Are Correlated with Slow Coronary Flow.Pubmed:27227272
  • Silencing salusin-β attenuates cardiovascular remodeling and hypertension in spontaneously hypertensive rats.pubmed:28230187
  • Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathypubmed:28333148
  • Salusin-β Is Involved in Diabetes Mellitus-Induced Endothelial Dysfunction via Degradation of Peroxisome Proliferator-Activated Receptor Gamma10.1155:2017
  • Silencing salusin β ameliorates heart failure in aged spontaneously hypertensive rats by ROS-relative MAPK/NF-κB pathways in the paraventricular nucleusPubmed: 30581101
  • Increased level of Plasma Salusin-α and Salusin-β in patients with Multiple SclerosisPubmed: 30738876
  • Salusin-α levels are negatively correlated with diastolic blood pressure in children with obesityPubmed: 31486354
  • Salusin-β mediates tubular cell apoptosis in acute kidney injury: Involvement of the PKC/ROS signaling pathwayPubmed: 31884071
  • Salusin-β is superior to salusin-α as a marker for evaluating coronary atherosclerosisPubmed: 32054363
  • A TOR2A Gene Product: Salusin-β Contributes to Attenuated Vasodilatation of Spontaneously Hypertensive Rats.Pubmed: 32458319
  • Serum level of salusin β as an indicator of metabolic disorders in Acute Lymphoblastic Leucaemia and Wilms Tumour survivors
  • Salusin‑β participates in high glucose‑induced HK‑2 cell ferroptosis in a Nrf‑2‑dependent manner34296310

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