ELISA Kit DIY Materials for Sex Hormone Binding Globulin (SHBG) Homo sapiens (Human) Assay Kit DIY Materials

ABP; TEBG; SBP; Sex steroid-binding protein; Testis-specific androgen-binding protein; Testosterone-estradiol-binding globulin; Testosterone-estrogen-binding globulin

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.62ng/mL
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  • ELISA Kit DIY Materials for Sex Hormone Binding Globulin (SHBG) Packages (Simulation)
  • ELISA Kit DIY Materials for Sex Hormone Binding Globulin (SHBG) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Sex Hormone Binding Globulin (SHBG). No significant cross-reactivity or interference between Sex Hormone Binding Globulin (SHBG) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • A Pilot Study Comparing the Effect of Flaxseed, Aromatase Inhibitor, and the Combination on Breast Tumor BiomarkersTandfonline: Source
  • Elevated Serum Bisphenol A Level in Patients with Dilated CardiomyopathyPubMed: 25996886
  • Effects of Fermented Dandelion (Taraxacum coreanum) Extract on Male Climacteric SyndromeSMGHBM_2016_v26n9_1063
  • Molecular characterization of insulin resistance and glycolytic metabolism in the rat uteruspubmed:27461373
  • Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome.pubmed:28336389
  • Progressive effects of silver nanoparticles on hormonal regulation of reproduction in male ratspubmed:27746313
  • 益肾祛浊方对PCOS-IR大鼠Ghrelin、SHBG及VF的影响yycyzx201733003
  • Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndromePubmed: 29535146
  • Comparative study on beneficial effects of vitamins B and D in attenuating doxorubicin induced cardiotoxicity in rats: Emphasis on calcium homeostasis34029952
  • Improvement of testosterone deficiency by fermented Momordica charantia extracts in aging male rats33868755
  • Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome MicePubmed:35057459

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