ELISA Kit DIY Materials for Sorbitol Dehydrogenase (SDH)

SORD; L-iditol 2-dehydrogenase

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,300.00 US$ 2,166.00 US$ 3,899.00 US$ 7,581.00 US$ 12,996.00
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Overview

Properties

Product No.KSB495Ra01
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only
Downloadn/a
CategoryEnzyme & Kinase Metabolic pathway

Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable SampleSerum, plasma, tissue homogenates, cell lysates and other biological fluids.
Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
Detectable Range1.56-100ng/mL
Applicable Sensitivity0.66ng/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Sorbitol Dehydrogenase (SDH). No significant cross-reactivity or interference between Sorbitol Dehydrogenase (SDH) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Citations

The Importance of Concentrations of Sorbitol Dehydrogenase and Glutamate Dehydrogenase and B-Mode Ultrasonographic Examination in The Diagnosis of Hepatic Lipidosis in Dairy CowsKafkas: Source
Grape seed extract and Zinc containing nutritional food supplement delays onset and progression of Streptozocin-induced diabetic cataract in Wistar ratsSpringer: Source
Aldo-keto reductase and sorbitol dehydrogenase enzymes in Egyptian diabetic patients with and without proliferative diabetic retinopathy.Pubmed: 23452182
Zinc/alogliptin combination attenuates testicular toxicity induced by doxorubicin in rats: Role of oxidative stress, apoptosis and TGF-β1/NF-κB signalingpubmed:29091894
Protective effects of N (2)‑L‑alanyl‑L‑glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusionPubmed:29393473
Effect of the combination between empagliflozin and calcipotriol on cadmium-induced testicular toxicity in rats