ELISA Kit DIY Materials for Stromal Cell Derived Factor 1 (SDF1) Homo sapiens (Human) Assay Kit DIY Materials

CXCL12; PBSF; P-BSF; SCYB12; SDF1-A; SDF1-B; SDF1A; SDF1B; TLSF-A; TLSF-B; TPAR1; Pre-B Cell Growth-Stimulating Factor; Chemokine C-X-C-Motif Ligand 12

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.057ng/mL
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  • ELISA Kit DIY Materials for Stromal Cell Derived Factor 1 (SDF1) Packages (Simulation)
  • ELISA Kit DIY Materials for Stromal Cell Derived Factor 1 (SDF1) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Stromal Cell Derived Factor 1 (SDF1). No significant cross-reactivity or interference between Stromal Cell Derived Factor 1 (SDF1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Endothelial progenitor cells (EPC) in sepsis with acute renal dysfunction (ARD)Goescholar: source
  • The promotion of endothelial progenitor cells recruitment by nerve growth factors in tissue-engineered blood vesselsScienceDirect: S0142961209012654
  • The use of BDNF to enhance the patency rate of small-diameter tissue-engineered blood vessels through stem cell homing mechanismsScienceDirect: S0142961211011422
  • Tanshinone IIA increases recruitment of bone marrow mesenchymal stem cells to infarct region via up-regulating stromal cell-derived factor-1/CXC chemokine receptor 4 axis in a myocardial ischemia modelScienceDirect: S0944711310003260
  • Monocyte/macrophages promote vasculogenesis in choroidal neovascularization in mice by stimulating SDF-1 expression in RPE cellsPubMed: 21655971
  • Pleiotropic beneficial effects of sonic hedgehog gene therapy in an experimental model of peripheral limb ischemiaPubMed: 21224834
  • Elevated PLGF contributes to small-cell lung cancer brain metastasisPubMed: 22797069
  • Impairment and Differential Expression of PR3 and MPO on Peripheral Myelomonocytic Cells with Endothelial Properties in Granulomatosis with PolyangiitisPubMed: 22792461
  • Serum levels of selected chemokines in systemic lupus erythematosus patientsPubmed: 22461186
  • Hyperglycemia promotes vasculogenesis in choroidal neovascularization in diabetic mice by stimulating VEGF and SDF-1 expression in retinal pigment epithelial cellsPubmed: 24780853
  • The non-alcoholic fraction of beer increases stromal cell derived factor 1 and the number of circulating endothelial progenitor cells in high cardiovascular risk subjects: A randomized clinical trialPubmed:24530788
  • Hypoxic exercise training improves cardiac/muscular hemodynamics and is associated with modulated circulating progenitor cells in sedentary menPubmed:24286591
  • Tissue Inhibitor of Metalloproteinase-1 and -3 Improves Cardiac Function in an Ischemic Cardiomyopathy Model RatPubmed:24814095
  • Comparison of chemokines (CCL-5 and SDF-1), chemokine receptors (CCR-5 and CXCR-4) and IL-6 levels in patients with different severities of depressionPubmed:25150002
  • Circulating CD133+ CD34+ Progenitor Cells and Plasma Stromal-Derived Factor-1Alpha: Predictive Role in Ischemic Stroke PatientsPubmed:25444027
  • The MCP-1, CCL-5 and SDF-1 chemokines as pro-inflammatory markers in generalized anxiety disorder and personality disordersPubmed:25560580
  • Stromal cell-derived factor 1 (SDF-1) accelerated skin wound healing by promoting the migration and proliferation of epidermal stem cellsPubmed:25636237
  • Serum concentrations of chemokines (CCL-5 and CXCL-12), chemokine receptors (CCR-5 and CXCR-4), and IL-6 in patients with posttraumatic stress disorder and avoidant personality disorderPubMed: 26481549
  • Stromal cell-derived factor 1 promoted migration of adipose-derived stem cells to the wounded area in traumatic ratsPubMed: 26392311
  • Role of SDF-1 and Wnt signaling pathway in the myocardial fibrosis of hypertensive ratsPubMed: 26396666
  • A Novel Combination of Calprotectin and CXCL13 for Predicting Malignancy in Patients with Exudative Pleural EffusionPubMed: 26632727
  • A study of chemokines, chemokine receptors and interleukin-6 in patients with panic disorder, personality disorders and their co-morbidityPubmed:27208878
  • Plasma Chemokines in Patients with Alcohol Use Disorders: Association of CCL11 (Eotaxin-1) with Psychiatric Comorbidity.pubmed:28149283
  • High-intensity Interval training enhances mobilization/functionality of endothelial progenitor cellsand depressed shedding of vascular endothelial cells undergoing hypoxia.pubmed:27761657
  • AMD3100 treatment attenuates pulmonary angiogenesis by reducing the c-kit (+) cells and its pro-angiogenic activity in CBDL rat lungspubmed:29233725
  • Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagensISSN:1936-2625/IJCEP0058424
  • c-Kit-Positive Adipose Tissue-Derived Mesenchymal Stem Cells Promote the Growth and Angiogenesis of Breast Cancerpubmed:28573141
  • Bone marrow-derived cells and their conditioned medium induce microvascular repair in uremic rats by stimulation of endogenous repair mechanisms.pubmed:28842629
  • Effects of the fibrous topography-mediated macrophage phenotype transition on the recruitment of mesenchymal stem cells: An in vivo study.pubmed:29017079
  • The effects of Sr-incorporated micro/nano rough titanium surface on rBMSC migration and osteogenic differentiation for rapid osteointegrationPubmed:29850672
  • Low‑intensity pulsed ultrasound promotes periodontal ligament stem cell migration through TWIST1‑mediated SDF‑1 expressionPubmed:29620151
  • β‐blockers interfere with cell homing receptors and regulatory proteins in a model of spontaneously hypertensive ratsPubmed:29752864
  • Mesenchymal stromal cell secretory factors induce sustained improvements in islet function pre-and post-transplantationDoi: 10.1016/j.jcyt.2018.07.007
  • Functionalization of Silk Fibroin Electrospun Scaffolds via BMSC Affinity Peptide Grafting through Oxidative Self-Polymerization of Dopamine for Bone RegenerationPubmed: 30777748
  • Stromal cell-derived factor-1α promotes recruitment and differentiation of nucleus pulposus-derived stem cellsPubmed: 30949297
  • Effects of Stromal Cell-Derived Factor-1α Secreted in Degenerative Intervertebral Disc on Activation and Recruitment of Nucleus Pulposus-Derived Stem CellsPubmed: 31827537
  • Short-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callusPubmed: 32894200
  • Enhanced recruitment and hematopoietic reconstitution of bone marrow‐derived mesenchymal stem cells in bone marrow failure by the SDF‐1/CXCR4Pubmed: 32633015
  • Highly interconnected inverse opal extracellular matrix scaffolds enhance stem cell therapy in limb ischemia33878473
  • bFGF and SDF-1¦Á Improve In Vivo Performance of VEGF-Incorporating Small-Diameter Vascular Grafts33800631
  • Combination of an engineered Lactococcus lactis expressing CXCL12 with light‐emitting diode yellow light as a treatment for scalded skin in mice34310856

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