ELISA Kit DIY Materials for Superoxide Dismutase 2, Mitochondrial (SOD2)
IPO-B; MNSOD; Mn-SOD
- UOM
- FOB US$ 1,088.00 US$ 1,813.00 US$ 3,263.00 US$ 6,346.00 US$ 10,878.00
- Quantity
Overview
Properties
- Product No.KSB083Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only - Downloadn/a
- CategoryEnzyme & KinaseMetabolic pathwayTumor immunityCardiovascular biologyNeuro science
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.78-50ng/mL
- Applicable Sensitivity0.37ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Superoxide Dismutase 2, Mitochondrial (SOD2). No significant cross-reactivity or interference between Superoxide Dismutase 2, Mitochondrial (SOD2) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Antioxidant profile of salivary glands in high fat diet-induced insulin resistance ratsPubmed: 24106991
- Consequences of age on ischemic wound healing in rats: altered antioxidant activity and delayed wound closurePubmed:24443098
- Determination of Gene Expression and Serum Levels of MnSOD and GPX1 in Colorectal CancerPubmed:25550558
- Antioxidant profile, carbonyl and lipid oxidation markers in the parotid and submandibular glands of rats in different periods of streptozotocin induced diabetesPubMed: 26143097
- Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human salivaPubMed: 26608886
- DDAH1 deficiency promotes intracellular oxidative stress and cell apoptosis via a miR-21-dependent pathway in mouse embryonic fibroblasts.Pubmed:26806551
- Cardiomyocyte dimethylarginine dimethylaminohydrolase1 attenuates left-ventricular remodeling after acute myocardial infarction: involvement in oxidative stress and …Pubmed:29892894
- Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndromePubmed: 30342783
- Selected elements of extracellular matrix of the skin in diabetes and insulin resistancePubmed: 31146169
- A Machine Learning-driven Study Indicates Emodin Improves Cardiac Hypertrophy by Modulation of Mitochondrial SIRT3 SignalingPubmed: 32135248
- Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease DevelopmentPubmed: 32751983
- Attenuation of Oxidative Stress and Inflammatory Response by Chronic Cannabidiol Administration Is Associated with Improved n-6/n-3 PUFA Ratio in the White and?¡34064937
- Serum Total SOD Activity and SOD1/2 Concentrations in Predicting All-Cause Mortality in Lung Cancer Patients34832849
- α-Lipoic acid ameliorates inflammation state and oxidative stress by reducing the content of bioactive lipid derivatives in the left ventricle of rats fed a high-fat dietPubmed:35569738