ELISA Kit DIY Materials for Syndecan 1 (SDC1) Mus musculus (Mouse) Assay Kit DIY Materials

CD138; SDC; SYND1; Syndecan Proteoglycan 1

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.625-40ng/mL
  • Applicable Sensitivity0.277ng/mL
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  • ELISA Kit DIY Materials for Syndecan 1 (SDC1) Packages (Simulation)
  • ELISA Kit DIY Materials for Syndecan 1 (SDC1) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Syndecan 1 (SDC1). No significant cross-reactivity or interference between Syndecan 1 (SDC1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Increased level of soluble syndecan-1 in serum correlates with myocardial expression in a rat model of myocardial infarctionSpringerLink: a201w3l18k257462
  • Sdc1 Overexpression Inhibits the p38 MAPK Pathway and Lessens Fibrotic Ventricular Remodeling in MI RatsSpringer: Source
  • Damage of the endothelial glycocalyx in chronic kidney diseasePubmed:24727235
  • Effect of valproic acid and injury on lesion size and endothelial glycocalyx shedding in a rodent model of isolated traumatic brain injuryPubmed:25058256
  • Impairment of the Endothelial Glycocalyx in Cardiogenic Shock and its Prognostic RelevancePubmed:25692257
  • Dexamethasone Suppressed LPS-Induced Matrix Metalloproteinase and Its Effect on Endothelial Glycocalyx SheddingPubMed: 26199464
  • Platelet-Derived Growth Factor-B Protects Rat Cardiac Allografts From Ischemia-reperfusion Injury.PubMed: 26371596
  • Dual antiplatelet and anticoagulant APAC prevents experimental ischemia–reperfusion-induced acute kidney injurypubmed:27405618
  • Endothelial glycocalyx layer shedding following lung resectionpubmed:27643669
  • Resuscitation with Pooled and Pathogen-Reduced Plasma Attenuates the Increase in Brain Water Content following Traumatic Brain Injury and Hemorrhagic Shock in Rats10.1089
  • Resuscitation with pooled and pathogen-reduced plasma attenuates the increase in brain water content following traumatic brain injury and hemorrhagic shock in rats the rat.doi/10.1089/neu.2016.4574
  • Volume kinetics of Ringer's lactate solution in acute inflammatory disease10.1016:j.bja.2018.04.023
  • MPO (myeloperoxidase) reduces endothelial glycocalyx thickness dependent on its cationic chargePubmed:29903730
  • Plasma resuscitation improved survival in a cecal ligation and puncture rat model of sepsisPubmed:28591008
  • Experimental models of endotheliopathy: impact of shock severityPubmed:28697004
  • Effects of propranolol and clonidine on brain edema, blood-brain barrier permeability, and endothelial glycocalyx disruption after fluid percussion brain injury in the ratPubmed:28930945
  • The Endothelial Glycocalyx in the Peritoneal Microcirculation of Rats with Chronic Kidney Failure Exposed to Dialysis Solutions173015_06.pdf
  • Plasma ameliorates endothelial dysfunction in burn injuryDoi: 10.1016/j.jss.2018.08.027
  • Dexmedetomidine preserves the endothelial glycocalyx and improves survival in a rat heatstroke modelPubmed: 30374889
  • Postoperative microcirculatory perfusion and endothelial glycocalyx shedding following cardiac surgery with cardiopulmonary bypassPubmed: 30687934
  • Microvascular alterations during cardiac surgery using a heparin or phosphorylcholine coated circuitPubmed: 31787433
  • Increased syndecan-1 and glypican-3 predict poor perinatal outcome and treatment resistance in intrahepatic cholestasis: Syndecan-1, glypican-3 and ICPPubmed: 31919038
  • Heparin Binding Protein and Endothelial Glycocalyx Markers in Severe COVID-19–A Prospective Observational Cohort Study
  • Newly Developed Recombinant Antithrombin Protects the Endothelial Glycocalyx in an Endotoxin-Induced Rat Model of Sepsis33375342
  • Effect of liraglutide on microcirculation in rat model with absolute insulin deficiency34119534
  • The effect of pre-operative methylprednisolone on postoperative delirium in elderly patients undergoing gastrointestinal surgery: a randomized, double-blind, placebo …34423832
  • Inhalation of 2% hydrogen improves survival rate and attenuates shedding of vascular endothelial glycocalyx in rats with heat stroke34524269
  • Dexmedetomidine suppresses serum syndecan-1 elevation and improves survival in a rat hemorrhagic shock modelPubmed:35110424
  • Resuscitation of hemorrhagic shock using normal saline does not damage the glycocalyx in the immediate resuscitation phase

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