ELISA Kit DIY Materials for Synuclein Alpha (SNCa) Mus musculus (Mouse) Assay Kit DIY Materials

SNC-A; aSYN; PD1; PARK1; PARK4; NACP; Non-A Beta Component Of Alzheimer's Disease Amyloid Precursor Protein; Non-A4 component of amyloid precursor

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range78-5000pg/mL
  • Applicable Sensitivity28pg/mL
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  • ELISA Kit DIY Materials for Synuclein Alpha (SNCa) Packages (Simulation)
  • ELISA Kit DIY Materials for Synuclein Alpha (SNCa) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Synuclein Alpha (SNCa). No significant cross-reactivity or interference between Synuclein Alpha (SNCa) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Cerebrospinal fluid–based kinetic biomarkers of axonal transport in monitoring neurodegenerationPubMed: PMC3428100
  • Lysosomal dysfunction increases exosome-mediated alpha-synuclein release and transmissionPubMed: PMC3107939
  • Parkinson's disease induced pluripotent stem cells with triplication of the α-synuclein locusPubMed: PMC3265381
  • Mutations of PARK Genes and Alpha-Synuclein and Parkin Concentrations in Parkinson’s DiseaseIntechopen:Source
  • Apolipoprotein Eε4: A Biomarker for Executive Dysfunction among Parkinson's Disease Patients with Mild Cognitive Impairmentpubmed:29326545
  • The anti-aging protein klotho alleviates injury of nigrostriatal dopaminergic pathway in 6-hydroxydopamine rat model of Parkinson's disease: Involvement of PKA/CaMKII/CREB signaling.pubmed:29107062
  • Development and biochemical characterization of a mouse model of Parkinson's disease bearing defective glucocerebrosidase activityPubmed: 30521842
  • Potential therapeutic effects of antagonizing adenosine A2A receptor, curcumin and niacin in rotenone-induced Parkinson's disease mice modelPubmed: 31820278
  • Nose to brain delivery of rotigotine loaded chitosan nanoparticles in human SH-SY5Y neuroblastoma cells and animal model of Parkinson's diseasePubmed: 32084576
  • Anti-α-synuclein ASO delivered to monoamine neurons prevents α-synuclein accumulation in a Parkinson's disease-like mouse model and in monkeysPubmed: 32810825

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