ELISA Kit DIY Materials for Thyroid Stimulating Hormone (TSH) Homo sapiens (Human) Assay Kit DIY Materials

Thyrotropin

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids。
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range246.9-20,000pg/mL
  • Applicable Sensitivity88.7pg/mL
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  • ELISA Kit DIY Materials for Thyroid Stimulating Hormone (TSH) Packages (Simulation)
  • ELISA Kit DIY Materials for Thyroid Stimulating Hormone (TSH) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Thyroid Stimulating Hormone (TSH). No significant cross-reactivity or interference between Thyroid Stimulating Hormone (TSH) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Alterations in the adenosine metabolism and CD39/CD73 adenosinergic machinery cause loss of Treg cell function and autoimmunity in ADA-deficient SCIDPubmed: 22184407
  • Can Nigella Sativa oil (NSO) reverse hypothyroid status induced by PTU in rat? biochemical and histological studiesLifesciencesite: Source
  • Impact of Metformin and Compound C on NIS expression and iodine uptake in vitro and in vivo: a role for CRE in AMPK modulation of thyroid functionPubmed: 23819433
  • Effect of lettuce biofortified with iodine by soil fertilization on iodine concentration in various tissues and selected biochemical parameters in serum of Wistar ratsSciencedirect:S1756464615000894
  • Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesisPubmed:24928937
  • Thyroid-stimulating hormone decreases HMG-CoA reductase phosphorylation via AMP-activated protein kinase in the liverPubMed: 25713102
  • Lead exposure causes thyroid abnormalities in diabetic ratsPubMed: 26221254
  • Perinatal Iron Deficiency-Induced Hypothyroxinemia Impairs Early Brain Development Regardless of Normal Iron Levels in the Neonatal BrainPubmed:27231981
  • The Impact of Carrot Enriched in Iodine through Soil Fertilization on Iodine Concentration and Selected Biochemical Parameters in Wistar RatsPubmed:27043135
  • Knock-In of the Recurrent R368X Mutation of PRKAR1A that Represses cAMP-Dependent Protein Kinase A Activation: A Model of Type 1 Acrodysostosis.pubmed:27589370
  • Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspringpubmed:27803384
  • Exposure of pregnant mice to triclosan impairs placental development and nutrient transport.pubmed:28322267
  • Endemic goitre in the Sudan despite longstanding programmes for the control of iodine deficiency disordershandle:123456789
  • Effect of Intracerebroventricular Administration of Apelin-13 on the Hypothalamus–Pituitary–Thyroid Axis and Peripheral Uncoupling Proteinsarticle:10.1007
  • Neuroprotective effects of thymoquinone against cerebellar histopathological changes in propylthiouracil-induced hypothyroidism in adult rats10.4314/tjpr.v16i5.9
  • The NANCI-Nkx2.1 gene duplex buffers Nkx2.1 expression to maintain lung development and homeostasis.pubmed:28546511
  • Voluntary exercise training restores anticontractile effect of NO in coronary arteries of adult rats with antenatal/early postnatal hypothyroidismPubmed:29307633
  • Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island populationPubmed:29525625
  • Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in MicePubmed:29403355
  • Central irisin administration suppresses thyroid hormone production but increases energy consumption in ratsPubmed:29574218
  • Hashimoto's thyroiditis induces neuroinflammation and emotional alterations in euthyroid micePubmed: 30373627
  • Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in MicePubmed: 30404008
  • The Adverse Effects of Thyrotropin Absence on Pancreatic β Cell Function in Mice
  • Hypothyroidism-related zinc deficiency leads to suppression of T lymphocyte activityPubmed: 31056723
  • Oxidative Stress Produced by Hyperthyroidism Status Induces the Antioxidant Enzyme Transcription through the Activation of the Nrf-2 Factor in Lymphoid Tissues of …
  • Hashimoto's thyroiditis impairs embryo implantation by compromising endometrial morphology and receptivity markers in euthyroid micePubmed: 31729993
  • Tanycyte ablation in the arcuate nucleus and median eminence increases obesity susceptibility by increasing body fat content in male micePubmed: 32173924
  • The role of protein disulphide-isomerase A3 as autoantigen in the pathogenesis of autoimmune thyroiditis and related brain damage in adult micePubmed: 31982645
  • Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axisPubmed: 33177714
  • Generation and Characterization of a New Resistance to Thyroid Hormone Mouse Model with Thyroid Hormone Receptor Alpha Gene MutationPubmed: 32924834
  • Hashimoto's thyroiditis induces hippocampus-dependent cognitive alterations by impairing astrocytes in euthyroid micePubmed: 32907517
  • Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathyPubmed: 32442899
  • Maternal exposure of triclosan causes fetal development restriction and female reproductive alterations in rat offspring/A exposição materna ao triclosan causa …
  • Comparaison des marqueurs hormonaux et histomorphométriques chez la souris un modèle d'hypothyroïdie après 28 jours de traitement au PTU par voie alimentaire.
  • Thyroid hormones, Sertoli cell proliferation and differentiation in progenies from carbamazepine©\treated rat dams during pregnancy and lactation33433934
  • Interplay between Thyroid Hormones and Stearoyl-CoA Desaturase 1 in the Regulation of Lipid Metabolism in the Heart33375430
  • Role of Kallikrein 7 in Body Weight and Fat Mass Regulation. Biomedicines 2021, 9, 13133572949
  • Maternal Subclinical Hypothyroidism in Rats Impairs Spatial Learning and Memory in Offspring by Disrupting Balance of the TrkA/p75 NTR Signal Pathway33966253
  • Genome?wide profiling of DNA methylation and gene expression unravel the epigenetic landscape in diabetes-related hypothyroidism34092241
  • Improved Therapeutic Efficiency against Obesity through Transdermal Drug Delivery Using Microneedle Arrays
  • Effects of iodine excess on serum thyrotropin-releasing hormone levels and type 2 deiodinase in the hypothalamus of Wistar rats34250878
  • Correction of a knock-in mouse model of acrodysostosis with gene therapy using a rAAV9-CAG-human PRKAR2A vector34599290
  • Plasmin activity promotes amyloid deposition in a transgenic model of human transthyretin amyloidosis34876572
  • The Deubiquitinase OTUB1 Is a Key Regulator of Energy MetabolismPubmed:35163456
  • Effects of meteorin-like hormone on endocrine function of hypothalamo-hypophysial system and peripheral uncoupling proteins in ratsPubmed:35332411

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