ELISA Kit DIY Materials for Troponin T Type 2, Cardiac (TNNT2) Mus musculus (Mouse) Assay Kit DIY Materials

CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range15.6-1,000pg/mL
  • Applicable Sensitivity5.7pg/mL
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  • ELISA Kit DIY Materials for Troponin T Type 2, Cardiac (TNNT2) Packages (Simulation)
  • ELISA Kit DIY Materials for Troponin T Type 2, Cardiac (TNNT2) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Troponin T Type 2, Cardiac (TNNT2). No significant cross-reactivity or interference between Troponin T Type 2, Cardiac (TNNT2) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Cardioprotective Effect of Sulphonated Formononetin on Acute Myocardial Infarction in RatsPubMed: 21232020
  • Cardioprotective Effect of SMND-309, A Novel Derivate of Salvianolic Acid B on Acute Myocardial Infarction in RatsWiley: source
  • Protective roles of Asperosaponin VI, a triterpene saponin isolated from Dipsacus asper Wall on acute myocardial infarction in ratsScienceDirect: S0014299909009984
  • The altered expression profile of microRNAs in cardiopulmonary bypass canine models and the effects of mir-499 on myocardial ischemic reperfusion injuryPubMed: PMC3694448
  • Salivary high‐sensitivity cardiac troponin T levels in patients with acute myocardial infarctionPubmed: 22834943
  • In vivo and in vitro cardioprotective effects of Panax quinquefolium 20 (S)-protopanaxadiol saponins (PQDS), isolated from Panax quinquefoliumPubmed: 23700796
  • The effects of caloric restriction and age on thyroid hormone signalling in the heart of ratsPl:Source
  • Cardiomyopathy reverses with recovery of liver injury, cholestasis and cholanemia in mouse model of biliary fibrosisPubmed:24330504
  • Cardioprotective Effect of Rhizomes of Acorus gramineus Against Isoproterenol-Induced Cardiac Damage in PigsPubmed:24420420
  • Glutathione administration reduces mitochondrial damage and shifts cell death from necrosis to apoptosis in ageing diabetic mice hearts during exercisePubmed:25039894
  • Crocin protects against doxorubicin-induced myocardial toxicity in rats through down-reCavia (Guinea pig )lation of inflammatory and apoptic pathwaysPubmed:26807765
  • The Cardenolide Glycoside Acovenoside A Affords Protective Activity in Doxorubicin-Induced Cardiotoxicity in MicePubmed:27247000
  • Troponins, heat shock proteins and glycogen phosphorylase BB in umbilical cord blood of complicated pregnancies.pubmed:27937003
  • Outer Balloon Ligation Increases Success Rate of Ischemia-Reperfusion Injury Model in Mice.pubmed:27907155
  • Rosuvastatin improves myocardial and neurological outcomes after asphyxial cardiac arrest and cardiopulmonary resuscitation in ratspubmed:28076830
  • Imatinib attenuates cardiac fibrosis by inhibiting platelet-derived growth factor receptors activation in isoproterenol induced modelpubmed:28570599
  • Cardioprotective effect of Notch signaling on the development of myocardial infarction complicated by diabetes mellituspubmed:29042932
  • Increases in plasma corin levels following experimental myocardial infarction reflect the severity of ischemic injuryPubmed: 30192780
  • Tissue suction-mediated gene transfer to the beating heart in micePubmed: 32027678
  • Hormonal Response to Incremental and Continuous Exercise in Cyclists with Left Ventricle HypertrophyPubmed: 32148580
  • Chlorogenic acid prevents acute myocardial infarction in rats by reducing inflammatory damage and oxidative stressPubmed: 33022535
  • Norepinephrine Leads to More Cardiopulmonary Toxicities than Epinephrine by Catecholamine Overdose in RatsPubmed: 32947820
  • Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice33526170
  • Pharmacological inhibition of arachidonate 12-lipoxygenase ameliorates myocardial ischemia-reperfusion injury in multiple species34536344
  • Development of a novel ssDNA aptamer targeting cardiac troponin I and its clinical applications34673993
  • Hydrogen therapy after resuscitation improves myocardial injury involving inhibition of autophagy in an asphyxial rat model of cardiac arrestPubmed:35495584

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