ELISA Kit DIY Materials for Trypsinogen Activation Peptide (TAP) Homo sapiens (Human) Assay Kit DIY Materials

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range123.5-10000pg/mL
  • Applicable Sensitivity54.1pg/mL
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  • ELISA Kit DIY Materials for Trypsinogen Activation Peptide (TAP) Packages (Simulation)
  • ELISA Kit DIY Materials for Trypsinogen Activation Peptide (TAP) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Trypsinogen Activation Peptide (TAP). No significant cross-reactivity or interference between Trypsinogen Activation Peptide (TAP) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Diabetes aggravates acute pancreatitis and inhibits pancreas regeneration in miceSpringerLink: x4l01435t34452jr
  • Prior peritoneal lavage with hot 0.9% saline induces HSP70 expression and protects against cerulein-induced acute pancreatitis in ratsPubmed: 23096089
  • Case report of mannose-binding lectin (MBL) deficiency and postoperative sepsis and coagulopathy in a patient following total pancreatectomy for chronic pancreatitis.Pubmed:25262717
  • A small molecule inhibitor of NFκB blocks ER stress and the NLRP3 inflammasome and prevents progression of pancreatitispubmed:27418337
  • Effects of Calcium Channel Blockers on Trypsinogen Activation and Severity of Cerulein-induced Acute Pancreatitis in RatsIJPS:Source
  • Investigation of non-pharmaceutical cures for acute pancreatitis induced by cerulein in rats
  • The Regulatory Effect of the Kinase Inhibitor PD98059 on Autophagic Flux During Trypsinogen Activation in Pancreatic Acinar CellsPubmed: 32011537

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