ELISA Kit DIY Materials for Tryptase (TPS)
TPSAB1; Tryptase Alpha/Beta 1; Tryptase Alpha II; Tryptase Beta I; Tryptase-I; Tryptase-II; Tryptase-III
- UOM
- FOB US$ 1,197.00 US$ 1,995.00 US$ 3,591.00 US$ 6,983.00 US$ 11,970.00
- Quantity
Overview
Properties
- Product No.KSB070Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - DownloadInstruction Manual
- CategoryEnzyme & KinaseInfection immunityRheumatology
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range15.6-1,000pg/mL
- Applicable Sensitivity5.5pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Tryptase (TPS)). No significant cross-reactivity or interference between Tryptase (TPS) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polypsPubMed: 22534535
- Improved liver function and relieved pruritus after 4-phenylbutyrate therapy in a patient with progressive familial intrahepatic cholestasis type 2Pubmed:24530123
- Mediators of Mast Cells in Bullous Pemphigoid and Dermatitis HerpetiformisPubmed:25400334
- A Novel Model of IgE-Mediated Passive Pulmonary Anaphylaxis in RatsPubmed:25541997
- Peritoneal mast cell degranulation and gastrointestinal recovery in patients undergoing colorectal surgeryPubmed:25677271
- Elevated Serum Tryptase and Endothelin in Patients with ST Segment Elevation Myocardial Infarction: Preliminary ReportPubMed: 26089601
- Tryptase and protease-activated receptor-2 stimulate scratching behavior in a murine model of ovalbumin-induced atopic-like dermatitisPubMed: 26049029
- Altered expression of IFN-λ2 in allergic airway disorders and identification of its cell originsJournals: Mi
- Altered Expression of IFN-2 in Allergic Airway Disorders and Identification of Its Cell OriginsPubmed:27057098
- An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis
- Dengue virus-elicited tryptase induces endothelial permeability and shockPubmed: 31265436
- Mast Cells in Peritoneal Fluid From Women With Endometriosis and Their Possible Role in Modulating Sperm FunctionPubmed: 31998139
- Biomarkers of Allergic Asthma and their Association with Serum Parameters
- Effectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity
- Serum CD203c+ Extracellular Vesicle Serves as a Novel Diagnostic and Prognostic Biomarker for Succinylated Gelatin Induced Perioperative Hypersensitive …34650557
- Mast Cell-Derived Proteases Induce Endothelial Permeability and Vascular Damage in Severe Fever with Thrombocytopenia SyndromePubmed:35612327