ELISA Kit DIY Materials for Tyrosine Hydroxylase (TH)
TYH; DYT5b; Tyrosine 3-Monooxygenase; Dystonia 14
- UOM
- FOB US$ 1,300.00 US$ 2,166.00 US$ 3,899.00 US$ 7,581.00 US$ 12,996.00
- Quantity
Overview
Properties
- Product No.KSB438Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - DownloadInstruction Manual
- CategorySignal transductionEnzyme & KinaseNeuro scienceHormone metabolism
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampletissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.78-50ng/mL
- Applicable Sensitivity
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Tyrosine Hydroxylase (TH). No significant cross-reactivity or interference between Tyrosine Hydroxylase (TH) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- 1, 25-dyhydroxyvitamin D3 Attenuates l-DOPA-Induced Neurotoxicity in Neural Stem CellsPubmed:25102940
- Genetic Diagnosis of Two Dopa-Responsive Dystonia Families by Exome SequencingPubmed:25181484
- Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat.pubmed:28199883
- Elevated blood plasma levels of epinephrine, norepinephrine, tyrosine hydroxylase, TGFβ1, and TNFα associated with high-altitude pulmonary edema in an Indian population.pubmed:27540296
- Silencing salusin-β attenuates cardiovascular remodeling and hypertension in spontaneously hypertensive rats.pubmed:28230187
- Vaccarin administration ameliorates hypertension and cardiovascular remodeling in renovascular hypertensive ratspubmed:28681939
- Multigenerational disruption of the thyroid endocrine system in marine medaka after a life-cycle exposure to perfluorobutanesulfonatePubmed:29565584
- Cilostazol Mediated Nurr1 and Autophagy Enhancement: Neuroprotective Activity in Rat Rotenone PD ModelPubmed:29429051
- Tetrabromobisphenol A caused neurodevelopmental toxicity via disrupting thyroid hormones in zebrafish larvaePubmed:29407805
- The Effect of Recombinant Tyrosine Hydroxylase Expression on the Neurogenic Differentiation Potency of Mesenchymal Stem CellsPubmed:29656620
- Montelukast attenuates rotenone-induced microglial activation/p38 MAPK expression in rats: Possible role of its antioxidant, anti-inflammatory and antiapoptotic effectsPubmed: 30222980
- Silencing salusin β ameliorates heart failure in aged spontaneously hypertensive rats by ROS-relative MAPK/NF-κB pathways in the paraventricular nucleusPubmed: 30581101
- Hydroxychloroquine antiparkinsonian potential: Nurr1 modulation versus autophagy inhibition
- Perfluoropolyether carboxylic acids (novel alternatives to PFOA) impair zebrafish posterior swim bladder development via thyroid hormone disruptionPubmed: 31733528
- Embryonic exposure to pentabromobenzene inhibited the inflation of posterior swim bladder in zebrafish larvaePubmed: 31935612
- Nose to brain delivery of rotigotine loaded chitosan nanoparticles in human SH-SY5Y neuroblastoma cells and animal model of Parkinson's diseasePubmed: 32084576
- Thyroid disruption and developmental toxicity caused by Cd2+ in Schizopygopsis younghusbandi larvaePubmed: 32344129
- Selection of Cells for Parkinson's Disease Cell-Therapy
- Effects of SiO2 nanoparticles on the uptake of tetrabromobisphenol A and its impact on the thyroid endocrine system in zebrafish larvae33385677
- Tocotrienols protect differentiated SH-SY5Y human neuroblastoma cells against 6-hydroxydopamine-induced cytotoxicity by ameliorating dopamine biosynthesis and …Pubmed:35065349
- Omarigliptin attenuates rotenone-induced Parkinson's disease in rats: Possible role of oxidative stress, endoplasmic reticulum stress and immune modulationPubmed:35439590
- Unravelling the neuroprotective mechanisms of carotenes in differentiated human neural cells: biochemical and proteomic approachesPubmed:35415676
- Parental and transgenerational impairments of thyroid endocrine system in zebrafish by 2, 4, 6-tribromophenol