ELISA Kit DIY Materials for Mitochondrial Uncoupling Protein 2 (UCP2)
SLC25A8; UCPH; Solute carrier family 25 member 8 ;Uncoupling Protein 2, Mitochondrial
- UOM
- FOB US$ 1,286.00 US$ 2,143.00 US$ 3,857.00 US$ 7,501.00 US$ 12,858.00
- Quantity
Overview
Properties
- Product No.KSC586Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only - Downloadn/a
- CategoryDevelopmental science
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleTissue homogenates, cell lysates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.156-10ng/mL
- Applicable Sensitivity0.056ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Uncoupling Protein 2, Mitochondrial (UCP2). No significant cross-reactivity or interference between Uncoupling Protein 2, Mitochondrial (UCP2) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- FM19G11 Favors Spinal Cord Injury Regeneration and Stem Cell Self-Renewal by Mitochondrial Uncoupling and Glucose Metabolism InductionPubMed: 22865656
- A 4-week consumption of medium roast and dark roast coffees affects parameters of energy status in healthy subjectsSciencedirect:S0963996914002403
- The effect of delivery type on uncoupling protein-2 levelsPubMed: 26479203
- Vitamin C and E chronic supplementation differentially affect hepatic insulin signaling in ratsPubmed:29291420
- Dose-dependent reproductive toxicity of sodium benzoate in male rats: Inflammation, oxidative stress and apoptosisPubmed: 32911041
- Vitamin B12 ameliorate Tramadol-induced oxidative stress, endocrine imbalance, apoptosis and NO/iNOS/NF-κB expression in Sprague Dawley through regulatory …34923198