ELISA Kit DIY Materials for Vanin 1 (VNN1) Homo sapiens (Human) Assay Kit DIY Materials

Pantetheinase; Pantetheine hydrolase; Tiff66; Vascular non-inflammatory molecule 1

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, urine, tissue homogenates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.059ng/mL
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  • ELISA Kit DIY Materials for Vanin 1 (VNN1) Packages (Simulation)
  • ELISA Kit DIY Materials for Vanin 1 (VNN1) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Vanin 1 (VNN1). No significant cross-reactivity or interference between Vanin 1 (VNN1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Vanin-1; a potential biomarker for nephrotoxicant-induced renal injuryScienceDirect: S0300483X11003234
  • Urinary vanin-1 as a novel biomarker for early detection of drug-induced acute kidney injuryAspet: 192807
  • Urinary vanin-1 as a novel biomarker for early detection of drug-induced acute kidney injury.PubMed: 22399813
  • Early detection of renal injury using urinary vanin-1 in rats with experimental colitisPubMed: 23307618
  • Effects of Exercise and Ferula Gummosa on Apelin of Cardiac and Kidney Tissues in L-NAME induced Hypertension in RatsJm.um: Source
  • Value of vanin-1 assessment in adult patients with primary immune thrombocytopeniaPubmed: 23534352
  • Early detection of renal injury using urinary vanin-1 in rats with experimental colitis.Pubmed: 23307618
  • Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty ratsSagepub:Source
  • The effect of activated charcoal on adenine-induced chronic renal failure in ratsPubmed:24412558
  • Early urinary biomarkers for renal tubular damage in spontaneously hypertensive rats on a high salt intakePubMed: 26376947
  • Citrate Attenuates Adenine-Induced Chronic Renal Failure in Rats by Modulating the Th17/Treg Cell BalancePubMed: 26253296
  • Early prediction of cisplatin-induced nephrotoxicity by urinary vanin-1 in patients with urothelial carcinomaPubmed:27317936
  • Nanosized titanium dioxide resulted in the activation of TGF-β/Smads/p38MAPK pathway in renal inflammation and fibration of micePubmed:26850371
  • Citrate attenuates vascular calcification in chronic renal failure rats.pubmed:28332248
  • Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats.pubmed:29027259
  • Gum Acacia Improves Renal Function and Ameliorates Systemic Inflammation, Oxidative and Nitrosative Stress in Streptozotocin-Induced Diabetes in Rats …Pubmed:29550811
  • Vanin-1 in Renal Pelvic Urine Reflects Kidney Injury in a Rat Model of HydronephrosisPubmed: 30332759
  • Involvement of Vanin-1 in Ameliorating Effect of Oxidative Renal Tubular Injury in Dahl-Salt Sensitive RatsPubmed: 31514290
  • Urinary vanin‐1 associated with chronic kidney disease in hypertensive patients: A pilot studyPubmed: 32869948
  • Association of urinary vanin©\1 with kidney function decline in hypertensive patients34029440
  • Plasma vanin-1 as a novel biomarker of sepsis for trauma patients: a prospective multicenter cohort study33624223

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