ELISA Kit DIY Materials for Vascular Cell Adhesion Molecule 1 (VCAM1) Oryctolagus cuniculus (Rabbit) Assay Kit DIY Materials

CD106; INCAM-100; L1CAM

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range31.25-2000pg/mL
  • Applicable Sensitivity14.2pg/mL
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  • ELISA Kit DIY Materials for Vascular Cell Adhesion Molecule 1 (VCAM1) Packages (Simulation)
  • ELISA Kit DIY Materials for Vascular Cell Adhesion Molecule 1 (VCAM1) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Vascular Cell Adhesion Molecule 1 (VCAM1). No significant cross-reactivity or interference between Vascular Cell Adhesion Molecule 1 (VCAM1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Changes in the vascular cell adhesion molecule-1, intercellular adhesion molecule-1 and c-reactive protein following administration of aqueous extract of piper sarmentosum on experimental.BioMed: 1476511X
  • Long-term ethanol consumption initiates atherosclerosis in rat aorta through inflammatory stress and endothelial dysfunctionScienceDirect: S1537189112000705
  • Resveratrol abrogates adhesion molecules and protects against TNBS-induced ulcerative colitis in rats.PubMed: 22029500
  • EFFECTS OF ADRIAMYCIN, CISPLATIN, AND 5-FLUOROURACIL ON THE TESTES OF ALBINO RATSResearchgate: Source
  • Active immunization against tumor necrosis factor-alpha decreases proinflammatory cytokines, oxidative stress mediators and adhesion molecules risk factors in streptozotocin-induced diabetic rats.Pubmed: 23859636
  • Increased monocyte adhesion by endothelial expression of VCAM-1 missense variation in vitro Pubmed: 24075742
  • Activation of receptor for advanced glycation end products contributes to aortic remodeling and endothelial dysfunction in sinoaortic denervated rats.Pubmed: 23880178
  • Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformationsPubmed: 24199192
  • PO-0699 Estimation Of Adhesion Molecules Intrauterine Growth Retardation (iugr) NewbornsBmj:Source
  • PO-0701 Causes Of Fetal Growth Restriction And Early Neonatal OutcomeBmj:Source
  • PO-0698 Neonatal Resuscitation Practices In The Delivery Room In India: An Audit Using VideographyBmj:Source
  • Insights into the antiatherogenic molecular mechanisms of andrographolide against?Porphyromonas gingivalis-induced atherosclerosis in rabbitsPubmed:25172523
  • Curcumin attenuates adhesion molecules and matrix metalloproteinase expression in hypercholesterolemic rabbitsPubmed:25282128
  • Effect of consumption of fresh and heated virgin coconut oil on the blood pressure and inflammatory biomarkers: An experimental study in Sprague Dawley ratsSciencedirect:S2090506814000268
  • Effect of magnesium sulfate and thyroxine on inflammatory markers in a rat model of hypothyroidismDoi: Abs
  • Effect of agmatine on experimental vascular endothelial dysfunctionPubMed: 26424770
  • Serum lipid profile and inflammatory markers in the aorta of cholesterol-fed rats supplemented with extra virgin olive oil, sunflower oils and oil-productsPubMed: 26401576
  • Comparative angioprotective effects of magnesium compoundsPubMed: 25127069
  • Diverse contribution of bone marrow-derived late-outgrowth endothelial progenitor cells to vascular repair under pulmonary arterial hypertension and arterial neointimal formationPubMed: 26231083
  • Effects of glucagon-like peptide-1 on advanced glycation endproduct-induced aortic endothelial dysfunction in streptozotocin-induced diabetic rats: possible roles of Rho kinase- and AMP kinase-mediated nuclear factor κB signaling pathwaysPubmed:26758998
  • A Therapeutic Insight of Niacin and Coenzyme Q10 Against Diabetic Encephalopathy in RatsPubmed:26867655
  • Changes in blood pressure, vascular reactivity and inflammatory biomarkers following consumption of heated corn oil.pubmed:29084680
  • Therapeutic potential of mesenchymal stem cells in acute kidney injury is affected by administration timingpubmed:28338909
  • Epigallocatechin‑3‑gallate attenuates neointimal hyperplasia in a rat model of carotid artery injury by inhibition of high mobility group box 1 expressionpubmed:28962112
  • Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosispubmed:28821422
  • BuShenKangShuai tablet reduces atherosclerotic lesion by improving blood lipids metabolism and inhibiting inflammatory response via TLR4 and NF-κB signaling pathwayjournals:1758383
  • Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosisS0021967317311858
  • Protective effect of high mobility group box-1 silence on diabetic retinopathy: an in vivo studyISSN:1936-2625/IJCEP0055881
  • Significance of Soluble Endoglin as a Possible Marker of Cardiovascular Disease in Type 2 Diabetic Patients315113405
  • The directional migration and differentiation of mesenchymal stem cells toward vascular endothelial cells stimulated by biphasic calcium phosphate ceramic10.1093/rb/rbx028
  • Vasculotide, an angiopoietin-1 mimetic, restores microcirculatory perfusion and microvascular leakage and decreases fluid resuscitation requirements in …Pubmed:28968277
  • Characterization of the rhesus macaque (Macaca mulatta) scrub typhus model: Susceptibility to intradermal challenge with the human pathogen Orientia …Pubmed:29522521
  • Nephroprotective effect of saxagliptin against gentamicin-induced nephrotoxicity, emphasis on anti-oxidant, anti-inflammatory and anti-apoptic effectsPubmed:30012474
  • Bushenkangshuai Tablet Reduces Atherosclerotic Lesion by Improving Blood Lipids Metabolism and Inhibiting Inflammatory Response via TLR4 and NF-κB Signaling …Pubmed:29619063
  • L-Carnitine Supplementation Increases Trimethylamine-N-Oxide but not Markers of Atherosclerosis in Healthy Aged WomenPubmed: 30485835
  • Intercellular adhesion molecule-1 (ICAM-1) associates with 24-hour ambulatory blood pressure variability in type 2 diabetes and controlsPubmed: 30716657
  • Effect of Radiation on the Expression of CVD-related miRNAs, Inflammation and Endothelial Dysfunction of HUVECsPubmed: 30711956
  • The cordycepin derivative IMM-H007 improves endothelial dysfunction by suppressing vascular inflammation and promoting AMPK-dependent eNOS activation in …Pubmed: 30826323
  • Anti-Inflammatory and Anti-Apoptotic Effects of Stybenpropol A on Human Umbilical Vein Endothelial CellsPubmed: 31671764
  • Vanadyl sulphate ameliorates biomarkers of endothelial injury and coagulation and thrombosis in a rat model of hyperglycaemiaPubmed: 31774317
  • The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese childrenPubmed: 31771561
  • Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep ApneaPubmed: 31882827
  • Endothelial epithelial sodium channel involves in high-fat diet-induced atherosclerosis in low-density lipoprotein receptor-deficient micePubmed: 33065235
  • Anti-Atherogenic Effects of Orlistat on Obesity-Induced Vascular Oxidative Stress Rat Model33562069
  • Dehydroepiandrosterone (DHEA) Improves the Metabolic and Haemostatic Disturbances in Rats with Male Hypogonadism
  • Circulating markers of endothelial activation in canine parvoviral enteritis

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