ELISA Kit DIY Materials for Vascular Endothelial Growth Factor C (VEGFC)
VEGF-C; Flt4-L; VRPL; Vascular Endothelial Growth Factor-Related Protein
- UOM
- FOB US$ 1,166.00 US$ 1,944.00 US$ 3,499.00 US$ 6,804.00 US$ 11,664.00
- Quantity
Overview
Properties
- Product No.KSA145Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only - Downloadn/a
- CategoryCytokineTumor immunity
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range15.6-1000pg/mL
- Applicable Sensitivity6.1pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Vascular Endothelial Growth Factor C (VEGFC). No significant cross-reactivity or interference between Vascular Endothelial Growth Factor C (VEGFC) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Alternatively activated RAW264. 7 macrophages enhance tumor lymphangiogenesis in mouse lung adenocarcinomaPubMed: 19241443
- Expression of COX-2 and VEGF-C in cholangiocarcinomas at different clinical and pathological stagesPubMed: 26125824
- Fenofibrate inhibits the expression of VEGFC and VEGFR-3 in retinal pigmental epithelial cells exposed to hypoxiaPubMed: 26622498
- IL-10 Indirectly Regulates Corneal Lymphangiogenesis and Resolution of Inflammation via MacrophagesPubMed: 26608451
- Interleukin-6 Induces Vascular Endothelial Growth Factor-C Expression via Src-FAK-STAT3 Signaling in Lymphatic Endothelial Cells.pubmed:27383632
- RNAi-mediated gene silencing of vascular endothelial growth factor C suppresses growth andinduces apoptosis in mouse breast cancer in vitro and in vivo.pubmed:27895746
- Characterization of isolated liver sinusoidal endothelial cells for liver bioengineeringPubmed:29582235
- Effects of diphyllin as a novel V-ATPase inhibitor on TE-1 and ECA-109 cellsPubmed:29328465
- Dynamic signature of lymphangiogenesis during acute kidney injury and chronic kidney diseasePubmed: 31019289
- Isotretinoin does not alter VEGF-A and VEGF-C levels: Do retinoids behave differently in dose-dependent and/or in vivo/in vitro conditions?Pubmed: 32722957