ELISA Kit DIY Materials for Vasoactive Intestinal Peptide (VIP) Rattus norvegicus (Rat) Assay Kit DIY Materials

PHM27; Intestinal peptide PHV-42; Peptide histidine valine 42; Peptide histidine methioninamide 27

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range12.35-1,000pg/mL
  • Applicable Sensitivity
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  • ELISA Kit DIY Materials for Vasoactive Intestinal Peptide (VIP) Packages (Simulation)
  • ELISA Kit DIY Materials for Vasoactive Intestinal Peptide (VIP) Packages (Simulation)
  • KSA380Ra11.jpg Picture
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Vasoactive Intestinal Peptide (VIP). No significant cross-reactivity or interference between Vasoactive Intestinal Peptide (VIP) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • VIP gene variants related to idiopathic pulmonary arterial hypertension in Chinese populationWiley: source
  • Low levels of vasoactive intestinal peptide are associated with Chagas disease cardiomyopathyPubmed: 23800435
  • CGRP and VIP Levels as Predictors of Efficacy of Onabotulinumtoxin Type A in Chronic MigraineOnlinelibrary: head.12372
  • Roles of sphincter of Oddi motility and serum vasoactive intestinal peptide, gastrin and cholecystokinin octapeptideNCBI: PMC4000510
  • Increased VIP levels in peripheral blood outside migraine attacks as a potential biomarker of cranial parasympathetic activation in chronic migrainePubmed:24847167
  • Clinical study of electro-acupuncture treatment with different intensities for functional constipation patientsPubmed:25318892
  • Aqueous Fraction of Beta vulgaris Ameliorates Hyperglycemia in Diabetic Mice due to Enhanced Glucose Stimulated Insulin Secretion, Mediated by Acetylcholine and GLP-1, and Elevated Glucose Uptake via Increased Membrane Bound GLUT4 TransportersPubmed:25647228
  • Aqueous Fraction ofPubMed: 25647228
  • Oral curcumin has anti-arthritic efficacy through somatostatin generationPubMed: 25836921
  • Effects of sphincter of Oddi motility on the formation of cholesterol gallstonesPubmed:27350732
  • Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitisPubmed:27156748
  • Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patientsPubmed:27250233
  • No Change in Interictal PACAP Levels in Peripheral Blood in Women With Chronic Migrainepubmed:27634731
  • Using the "target constituent removal combined with bioactivity assay" strategy to investigate the optimum arecoline content in charred areca nut.pubmed:28054652
  • [6]-Gingerol, from Zingiber officinale, potentiates GLP-1 mediated glucose-stimulated insulin secretion pathway in pancreatic β-cells and increases RAB8/RAB10-regulated membrane presentation of GLUT4 transporters in skeletal muscle to improve hyperglycemia in Leprdb/db type 2 diabetic mice10.1186/s12906-017-1903-0
  • Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation10.1007/s13765-017-0327-3
  • Salivary neuropeptides, stress, and periodontitisPubmed:28914594
  • Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile AcidsPubmed:29853946
  • Vasoactive intestinal peptide degradation might influence Interleukin-17 expression in cardiac chagasic patientsPubmed: 30365640
  • Serum Levels of Neuropeptides in Cows with Left Abomasal DisplacementPubmed: 30562932
  • Protective Effect of Silkworm Pupa Oil on Hydrochloric Acid/Ethanol‐Induced Gastric UlcerPubmed: 30479041
  • Serum CGRP, VIP, and PACAP usefulness in migraine: a case–control study in chronic migraine patients in real clinical practicePubmed: 32951099
  • Disrupted local innervation results in less VIP expression in CF mice tissuesPubmed: 32600901
  • Anti-Inflammatory and Pro-Inflammatory Neuropeptides in Dilative Cardiomyopathy are there Relations to Psychophysical Stress?
  • Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome
  • VIP reduction in the pancreas of F508del homozygous CF mice and early signs of Cystic Fibrosis Related Diabetes (CFRD)34034984
  • Significance of serum VIP and PACAP in multiple sclerosis: an exploratory case–control study34698942
  • A clinical analysis on functioning pancreatic neuroendocrine tumors (focusing on VIPomas): a single-center experiencePubmed:35644576

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