ELISA Kit DIY Materials for Vimentin (VIM)

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,197.00 US$ 1,995.00 US$ 3,591.00 US$ 6,983.00 US$ 11,970.00
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Overview

Properties

Product No.KSB040Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only
Downloadn/a
CategoryTumor immunity Developmental science

Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable SampleSerum, plasma and other biological fluids.
Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
Detectable Range125-8000pg/mL
Applicable Sensitivity46pg/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Vimentin (VIM). No significant cross-reactivity or interference between Vimentin (VIM) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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ELISA / CLIA Experiment Service

Increment services

Citations

Aberrant Vimentin Methylation Is Characteristic of Breast Cancerpubmed:22315367
Glyoxalase 1-knockdown in human aortic endothelial cells - effect on the proteome andendothelial function estimates.pubmed:27898103
HtrA3 is a cellular partner of cytoskeleton proteins and TCP1α chaperoninPubmed:29477555
Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathyPubmed: 31511328
Extracellular vesicles derived from injured vascular tissue promote the formation of tertiary lymphoid structures in vascular allograftsPubmed: 31729155
Is Vimentin the Cause or Effect of Obstructive Sleep Apnea Development?Pubmed: 32088750
ZNF471 modulates EMT and functions as methylation regulated tumor suppressor with diagnostic and prognostic significance in cervical cancer33566221
Metastatic suppression by DOC2B is mediated by inhibition of epithelial-mesenchymal transition and induction of senescence33758996
Comparison of Vimentin Levels Between Preeclamptic and Normotensive Pregnant Women
Advanced glycation end-products associate with podocytopathy in type II diabetic patients
Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β …