ELISA Kit DIY Materials for Visfatin (VF) Homo sapiens (Human) Assay Kit DIY Materials

Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range1.23-100ng/mL
  • Applicable Sensitivity
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  • ELISA Kit DIY Materials for Visfatin (VF) Packages (Simulation)
  • ELISA Kit DIY Materials for Visfatin (VF) Packages (Simulation)
  • KSA638Hu11.jpg Picture
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Visfatin (VF). No significant cross-reactivity or interference between Visfatin (VF) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • EFFECT OF 8 WEEKS ENDURANCE TRAINING ON PLASMA VISFATIN IN MIDDLE-AGED MENBrjb: source
  • The relationship between visfatin and HOMA-IR in hypertensive patients, and the effect of antihypertensive drugs on visfatin and HOMA-IR in hypertensive patients with insulin resistanceScienceDirect: S0168822711002956
  • Early-effect of bariatric surgery (Scopinaro method) on intestinal hormones and adipokines in insulin resistant Wistar rat.Pubmed: 24304571
  • CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytesPubmed:25168658
  • Plasma visfatin levels and mRNA expression of visfatin in peripheral blood mononuclear cells and peripheral blood monocyte-derived macrophages from normal weight females with polycystic ovary syndromePubmed:Pmc3991522
  • Visfatin derived from ascites promotes ovarian cancer cell migration through Rho/ROCK signaling-mediated actin polymerization.Pubmed:25055182
  • Visfatin −948G/T and resistin −420C/Gpolymorphisms in Egyptian type 2 diabetic patients with and without cardiovascular diseasesNrcresearchpress:Source
  • Antidiabetic Effect of Galantamine: Novel Effect for a Known Centrally Acting DrugPubMed: 26262991
  • Changes in concentration of visfatin during four weeks of inpatient treatment of alcohol dependent malesScience: Article
  • Variation in blood levels of hormones in obese patients following weight reduction induced by endoscopic and surgical bariatric therapiesPubMed: 26539806
  • Genetic Variations in Visfatin and Resistin Genes in Egyptian Type 2 Diabetic Patients With and Without ComplicationsPubMed: 25120107
  • Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokinesscience:S0065128116301234
  • Changes in Serum Nampt Levels and Its Significances in Diabetic Nephropathy Patients-The Potential Role of Nampt in T2DM with Diabetic Nephropathy.pubmed:28699485
  • Post-chemotherapy serum anti-müllerian hormone level predicts ovarian function recoveryPubmed:30012648
  • Effect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal ratsPubmed:29784871
  • Hyperinsulinemic-euglycemic clamp over the first year of use of depot-medroxyprogesterone acetate as a contraceptivePubmed:29665358
  • Analysis of synovial fluid visfatin level in temporomandibular joint disordersPubmed:29385907
  • Resistin and visfatin concentrations are related to central obesity and inflammation in Brazilian children10.1186:s41110-018-0060-7
  • Transcriptional Regulation of NAMPT Gene by Glycogen Synthase Kinase 3β in Goat AdipocytesPubmed: 30615481
  • The Effect of 8 Weeks Aquatic Exercise Program on Plasma Visfatin Level and Some Blood Factors in Obese Men
  • Adipokines as Biomarkers in Health and Disease
  • Plasma concentration and expression of adipokines in epicardial and subcutaneous adipose tissue are associated with impaired left ventricular filling patternPubmed: 31533725
  • Relationshıp of spontaneous subarachnoid haemorrhage and cerebral aneurysm to serum Visfatin and Nesfatin-1 levelsPubmed: 32311618
  • Effects of 10-Week Concurrent Training on Insulin Resistance and the Serum Levels of Vaspin and Visfatin in Overweight Females
  • Different Ratios of Corn and Coconut Oil Blends in High©\Fat Diets Influence Fat Deposition without Altering Metabolic Biomarkers in Male Rats

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