ELISA Kit DIY Materials for Vitamin B1 (VB1)
Thiamine; Thiamin; Aneurine
- UOM
- FOB US$ 1,469.00 US$ 2,448.00 US$ 4,406.00 US$ 8,568.00 US$ 14,688.00
- Quantity
Overview
Properties
- Product No.KSD053Ge11
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- Category
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma and other biological fluids
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range3.70-300ng/mL
- Applicable Sensitivity1.63ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Vitamin B1 (VB1). No significant cross-reactivity or interference between Vitamin B1 (VB1) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Identification of critical variants within SLC44A4, an ulcerative colitis susceptibility gene identified in a GWAS in north IndiansPubmed:26741288
- 日粮不同钴水平对奶牛泌乳性能, 营养物质消化率和血液生化指标的影响
- Disinhibition-Like Behavior Correlates with Frontal Cortex Damage in an Animal Model of Chronic Alcohol Consumption and Thiamine DeficiencyPubmed:35203470