ELISA Kit DIY Materials for Vitamin D Receptor (VDR) Mus musculus (Mouse) Assay Kit DIY Materials

NR1I1; Nuclear Receptor Subfamily 1 Group I Member 1; Calcitriol Receptor; 1,25-dihydroxyvitamin D3 receptor

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.312-20ng/mL
  • Applicable Sensitivity0.113ng/mL
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  • ELISA Kit DIY Materials for Vitamin D Receptor (VDR) Packages (Simulation)
  • ELISA Kit DIY Materials for Vitamin D Receptor (VDR) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Vitamin D Receptor (VDR). No significant cross-reactivity or interference between Vitamin D Receptor (VDR) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • The vitamin D receptor: A therapeutic target for the treatment of breast cancer?Cgi: Content
  • Vitamin D receptor as a target for breast cancer therapy.pubmed:28213567
  • Effect of exercise on serum vitamin D and tissue vitamin Dreceptors in experimentally induced type 2 Diabetes Mellitus.pubmed:27504197
  • Vitamin D supplementation attenuates oxidative stress in paraspinal skeletal muscles in patients with low back painPubmed:29143122
  • Enhanced remedial effects for vitamin D3 and calcium co-supplementation against pre-existing lead nephrotoxicity in mice: The roles of renal calcium homeostatic …Pubmed: 30553018
  • Seluang Fish (Rasbora Spp.) Oil Decreases Inflammatory Cytokines Via Increasing Vitamin D Level in Systemic Lupus Erythematosus
  • Status of vitamin D and the associated host factors in pulmonary tuberculosis patients and their household contacts: a cross sectional studyPubmed: 31255688
  • Association of Fok1 VDR polymorphism with Vitamin D and its associated molecules in pulmonary tuberculosis patients and their household contactsPubmed: 31649297
  • Vitamin D Status in Dupuytren's Disease: Association with Clinical Status and Vitamin D Receptor Expression
  • Betok Fish (Anabas testudineus) Oil Decreases Inflammatory Cytokine through Increasing Vitamin D Level in Rats-induced Systemic Lupus Erythematosus
  • Positive Correlation between Monocyte-to-Lymphocyte Ratio and C-Reactive Protein in Vitamin D Deficient Preterm Infants with Respiratory Distress Syndrome
  • Identification of SNP of VDR and VDBP gene and its Dysregulated pathway through VDR-VDBP interaction network analysis in Vitamin D deficient Infertile …

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