ELISA Kit DIY Materials for Vitamin D2 (VD2)
Ergocalciferol; Deltalin; Drisdol; Calcidol
- UOM
- FOB US$ 1,433.00 US$ 2,388.00 US$ 4,298.00 US$ 8,358.00 US$ 14,328.00
- Quantity
Overview
Properties
- Product No.KSA921Ge11
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryMetabolic pathwayNeuro scienceNutrition metabolismBone metabolism
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma and other biological fluids.
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range1.23-100ng/mL
- Applicable Sensitivity0.53ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Vitamin D2 (VD2). No significant cross-reactivity or interference between Vitamin D2 (VD2) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Carbon dots-modified chitosan based electrochemical biosensing platform for detection of vitamin DPubmed:29275197
- Studies on Carbon quantum dots embedded Iron Oxide Nanoparticles and their Electrochemical responsePubmed: 32396882