ELISA Kit DIY Materials for Von Willebrand Factor (vWF) Rattus norvegicus (Rat) Assay Kit DIY Materials

F8VWF; VWD; von Willebrand antigen 2

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SamplePlasma.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range12.35-1000ng/mL
  • Applicable Sensitivity5.23ng/mL
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  • ELISA Kit DIY Materials for Von Willebrand Factor (vWF) Packages (Simulation)
  • ELISA Kit DIY Materials for Von Willebrand Factor (vWF) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Von Willebrand Factor (vWF). No significant cross-reactivity or interference between Von Willebrand Factor (vWF) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Amorphous silica nanoparticles impair vascular homeostasis and induce systemic inflammationPubmed:Pmc4047982
  • Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous CrisisPubmed:25448261
  • Short-Term Nose-Only Water-Pipe (Shisha) Smoking Exposure Accelerates Coagulation and Causes Cardiac Inflammation and Oxidative Stress in MicePubmed:25634761
  • Blood pH in coronary artery microthrombosis of ratsPubMed: 26522304
  • Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal InstillationPubMed: 25580880
  • Differential integrative omic analysis for mechanism insights and biomarker discovery of abnormal Savda syndrome and its unique Munziq prescriptionPubmed:27296761
  • Circulating endothelial cells, circulating endothelial progenitor cells, and von Willebrand factor in pregnancies complicated by hypertensive disordersdoi:10.1111
  • Low-Dose Sodium Nitrite Fluid Resuscitation Prevents Lethality From Crush Syndrome by Improving Nitric Oxide Consumption and Preventing Myoglobin Cytotoxicity in Kidney in A Rat Model.pubmed:27941593
  • The directional migration and differentiation of mesenchymal stem cells toward vascular endothelial cells stimulated by biphasic calcium phosphate ceramic10.1093/rb/rbx028
  • Early prognostic factors in septic shock cancer patients: a prospective study with a proteomic approachPubmed:29315472
  • Relationship between the von Willebrand Factor Plasma Concentration and Ultrasonographic Doppler Findings in Pregnancies Complicated by Hypertensive …Pubmed:29621786
  • Role of Pigment Epithelium-Derived Factor in Arsenic-Induced Vascular Endothelial Dysfunction in a Rat ModelPubmed: 30392020
  • Risk stratification and prognostic evaluation of endothelial cell‑specific molecule1, von Willebrand factor, and a disintegrin‑like and metalloprotease with …
  • Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep ApneaPubmed: 31882827
  • Waterpipe Tobacco Smoke Inhalation Triggers Thrombogenicity, Cardiac Inflammation and Oxidative Stress in Mice: Effects of FlavouringPubmed: 32075078
  • Pigment epithelium-derived factor (PEDF) ameliorates arsenic-induced vascular endothelial dysfunction in rats and toxicity in endothelial EA. hy926 cellsPubmed: 32315827
  • Xiaoyukang Jiaonang Promotes the Degradation of Hypoxia-Inducible Factor 1α and Antiangiogenesis and Anti-Inflammation in Chronic Subdural Hematoma …Pubmed: 32228124
  • Enhanced Fibrin-Lysis in Grade-1 Dengue Haemorrhagic Fever
  • Ad hatoda Vasica: a potential ayurvedic intervention against COVID-19 associated impaired immune response and hypoxia-inflammation phenotype
  • Investigating the Multi-Faceted Nature of Radiation-Induced Coagulopathies in a G?ttingen Minipig Model of Hematopoietic Acute Radiation Syndrome34019667
  • Preservation of renal endothelial integrity and reduction of renal edema by aprotinin does not preserve renal perfusion and function following experimental …34169407
  • Salvianolic acid B improves the survival rate, acute kidney dysfunction, inflammation and NETosis‑mediated antibacterial action in a crush syndrome rat modelPubmed:35386617

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