Anti-Actin Alpha 2, Smooth Muscle (ACTa2) Monoclonal Antibody

ACT-A2; ACTSA; ACTVS; Asma; GIG46; Cell growth-inhibiting gene 46 protein

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 119.00 US$ 279.00 US$ 398.00 US$ 995.00 US$ 3,980.00
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Overview

Properties

Product No.CAB342Hu22
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsLoading Control of WB
Research use only
DownloadInstruction Manual
CategoryDevelopmental science Bone metabolism

PurificationProtein A + Protein G affinity chromatography
LabelNone
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid, Concentration 1mg/ml

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Samples: Porcine Small intestine Tissue; Primary Ab: 20μg/ml Mouse Anti-Human ACTa2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Samples: Porcine Colon Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human ACTa2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Thyroid cancer Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human ACTa2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Rectum Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human ACTa2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Mouse Cardiac Muscle Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human ACTa2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Mouse Colon Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human ACTa2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Porcine Colon Tissue
Primary Ab: 20µg/ml Mouse Anti-Human ACTa2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Porcine Small intestine Tissue
Primary Ab: 20µg/ml Mouse Anti-Human ACTa2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Mouse Colon Tissue
Primary Ab: 20µg/ml Mouse Anti-Human ACTa2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Cy3 staining on IF;
Sample: Primary Rabbit Ureteral Smooth Muscle Cells cell
Primary Ab: 20µg/ml Mouse Anti-Human ACTa2 Antibody
Second Ab: 2µg/ml Cy3-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu16)
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 30µg/ml Mouse Anti-Human ACTa2 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)

Western Blot; Sample: Lane1: Rat Heart lysate; Lane2: Rat Cerebrum lysate; Lane3: Rat Skeletal muscle lysate Primary Ab: 2µg/ml Mouse Anti-Human ACTa2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
Western Blot; Samples: Lane1: A549 cell lysate; Lane2: Rat Heart lysate; Lane3: Porcine Heart lysate;
Primary Ab: 0.01µg/ml Mouse Anti-Human ACTa2 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against ACTa2. It has been selected for its ability to recognize ACTa2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-5µg/ml:1:500-5000
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Giveaways

Increment services

Citations

Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonicaPubmed:27157496
Effects of repeated increasing doses of cisplatin as models of acute kidney injury and chronic kidney disease in ratsPubmed: 32936352
Hesperidin inhibits the epithelial to mesenchymal transition induced by transforming growth factor-β1 in A549 cells through Smad signaling in the cytoplasm
Structural Changes and Detection of Liver Fibrosis¨CRelated Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate cells
Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate …34857049
NOX5 mediates the crosstalk between tumor cells and cancer‐associated fibroblasts via regulating cytokine network34459125