Anti-Actin Alpha 2, Smooth Muscle (ACTa2) Monoclonal Antibody Homo sapiens (Human) Protein, Antibody, Assaykit, Service

ACT-A2; ACTSA; ACTVS; Asma; GIG46; Cell growth-inhibiting gene 46 protein

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Overview
Properties
  • Ig Isotype IgG, Clone Number G5
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Excitation Laser
  • Vol. per test
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
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  • Anti-Actin Alpha 2, Smooth Muscle (ACTa2) Monoclonal Antibody Packages (Simulation)
  • Anti-Actin Alpha 2, Smooth Muscle (ACTa2) Monoclonal Antibody Packages (Simulation)
  • Anti-Actin Alpha 2, Smooth Muscle (ACTa2) Monoclonal Antibody Human HepG2 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human ACTa2 Monoclonal Antibody (Catalog FAB342Hu02, red histogram) or Isotype control antibody (Catalog IS067-Mu01, green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
  • Anti-Actin Alpha 2, Smooth Muscle (ACTa2) Monoclonal Antibody Human Jurkat cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human ACTa2 Monoclonal Antibody (Catalog FAB342Hu02, red histogram) or Isotype control antibody (Catalog IS067-Mu01, green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Usage

Western blotting: 0.2-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:10μg/ml;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Giveaways

Citations

  • Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonicaPubmed:27157496
  • Effects of repeated increasing doses of cisplatin as models of acute kidney injury and chronic kidney disease in ratsPubmed: 32936352
  • Hesperidin inhibits the epithelial to mesenchymal transition induced by transforming growth factor-β1 in A549 cells through Smad signaling in the cytoplasm
  • Structural Changes and Detection of Liver Fibrosis¨CRelated Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate cells
  • Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate …34857049
  • NOX5 mediates the crosstalk between tumor cells and cancer‐associated fibroblasts via regulating cytokine network34459125

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