Anti-Actin Alpha 2, Smooth Muscle (ACTa2) Polyclonal Antibody Homo sapiens (Human) Control antibody

ACT-A2; ACTSA; ACTVS; Asma; GIG46; Cell growth-inhibiting gene 46 protein

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Overview
Properties
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Anti-Actin Alpha 2, Smooth Muscle (ACTa2) Polyclonal Antibody Packages (Simulation)
  • Anti-Actin Alpha 2, Smooth Muscle (ACTa2) Polyclonal Antibody Packages (Simulation)
  • Anti-Actin Alpha 2, Smooth Muscle (ACTa2) Polyclonal Antibody DAB staining on IHC-P; Samples: Human Small intestine Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human ACTa2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Anti-Actin Alpha 2, Smooth Muscle (ACTa2) Polyclonal Antibody DAB staining on IHC-P;
    Samples: Human Stomach Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human ACTa2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Anti-Actin Alpha 2, Smooth Muscle (ACTa2) Polyclonal Antibody DAB staining on IHC-P;
    Sample: Human Kidney Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Human ACTa2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Anti-Actin Alpha 2, Smooth Muscle (ACTa2) Polyclonal Antibody DAB staining on IHC-P;
    Sample: Human Stomach Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Human ACTa2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • CAB342Hu01.jpg Figure. Western Blot; Sample: Recombinant ACTa2, Human.
  • Anti-Actin Alpha 2, Smooth Muscle (ACTa2) Polyclonal Antibody Western Blot; Samples: Lane1: Mouse Heart lysate; Lane2: Mouse Skeletal muscle lysate; Lane3: Rat Heart lysate; Lane4: Rat Skeletal muscle lysate; Lane5: Porcine Heart lysate; Lane6: Porcine Skeletal muscle lysate;
    Primary Ab: 0.005µg/ml Rabbit Anti-Human ACTa2 Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against ACTa2. It has been selected for its ability to recognize ACTa2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2ug/ml
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonicaPubmed:27157496
  • Effects of repeated increasing doses of cisplatin as models of acute kidney injury and chronic kidney disease in ratsPubmed: 32936352
  • Hesperidin inhibits the epithelial to mesenchymal transition induced by transforming growth factor-β1 in A549 cells through Smad signaling in the cytoplasm
  • Structural Changes and Detection of Liver Fibrosis¨CRelated Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate cells
  • Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate …34857049
  • NOX5 mediates the crosstalk between tumor cells and cancer‐associated fibroblasts via regulating cytokine network34459125

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