Anti-Beta Actin (ACTB) Monoclonal Antibody

Actin Beta; ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 119.00 US$ 279.00 US$ 398.00 US$ 995.00 US$ 3,980.00
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Overview

Properties

Product No.CAB340Hu22
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsLoading Control of WB
Research use only
DownloadInstruction Manual
CategorySignal transduction Developmental science Bone metabolism

PurificationProtein A + Protein G affinity chromatography
LabelNone
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 1mg/mL

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Packages (Simulation)
Packages (Simulation)

FITC staining on IF; Samples: Human MCF7 Cells; Primary Ab: 30µg/ml Mouse Anti-Human ACTb Antibody Second Ab: 1µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu18)
DAB staining on IHC-P;
Sample: Human Colorectal cancer Tissue;
Primary Ab: 10ug/ml Mouse Anti-Human ACTB Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Cerebrum Tissue;
Primary Ab: 10ug/ml Mouse Anti-Human ACTB Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Mouse Lung Tissue;
Primary Ab: 5ug/ml Mouse Anti-Human GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Mouse Testis Tissue;
Primary Ab: 5ug/ml Mouse Anti-Human GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Mouse Ovary Tissue;
Primary Ab: 5ug/ml Mouse Anti-Human GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Rat Pancreas Tissue;
Primary Ab: 5ug/ml Mouse Anti-Human GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Rat Liver Tissue;
Primary Ab: 5ug/ml Mouse Anti-Human GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Rat Skeletal muscle Tissue;
Primary Ab: 5ug/ml Mouse Anti-Human GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Rat Cerebrum Tissue;
Primary Ab: 5ug/ml Rabbit Anti-Human ACTb Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Cerebellum Tissue;
Primary Ab: 5ug/ml Rabbit Anti-Human ACTb Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Western Blot; Sample: Human Hela cell lysate; Primary Ab: 3µg/ml Mouse Anti-Human ACTb Antibody Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
Western Blot; Sample: Lane1: DU145 cell lysate; Lane2: OS-RC-2 cell lysate; Lane3: Rat Liver lysate; Lane4: Bovine Kidney lysate
Primary Ab: 0.03µg/ml Mouse Anti-Human ACTB Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot Sample: Lane1: Human Lung lysate; Lane2: 293T cell lysate; Lane3: Rat Liver lysate; Lane4: Rat Kidney lysate; Lane5: Porcine Liver lysate; Lane6: Porcine Kidney lysate;
Primary Ab: 1μg/ml Mouse Anti-Human ACTb Antibody
Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot Sample: Lane1: Mouse Liver lysate; Lane2: Mouse Kidney lysate; Lane3: Rabbit Liver lysate; Lane4: Bovine Kidney lysate; Lane5: Canine Kidney lysate;
Primary Ab: 1μg/ml Mouse Anti-Human ACTb Antibody
Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot; Sample: Human A549 cell lysate;
Primary Ab: 3µg/ml Mouse Anti-Human ACTb Antibody
Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot; Sample: Lane1: 293T cell lysate; Lane2: Hela cell lysate
Primary Ab: 0.01µg/ml Rabbit Anti-Human ACTB Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against ACTB. It has been selected for its ability to recognize ACTB in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.0003-0.1ug/ml
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Cavia (Guinea pig ), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Ovis aries; Ovine (Sheep), Equus caballus; Equine (Horse), Chicken (Gallus)

Giveaways

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Citations

Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and L-Glutamine on Molecular Responses in Chicken Embryo Pectoral MusclesNCBI:PMC3856104
Expression of chloride channels in mouse skin incisional wounds96
Targeted Elimination of Senescent Beta Cells Prevents Type 1 Diabetes
Neuroinflammation, Oxidative Stress, and Neurogenesis in a Mouse Model of Chronic Fatigue Syndrome, and the Treatment with Kampo MedicinePubmed: 31902915
Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor …Pubmed: 32046779
Effect of ischemic postconditioning on cell apoptosis and expression of relevant genes in non-culprit coronary arteriesPubmed: 32784207
Malted Barley Improved the Structure and Function of Gastrocnemius Muscle of Hypercholesterolemic Mother Rats and their Offspring
Tryptophan Depletion Modulates Tryptophanyl-tRNA Synthetase-Mediated High-Affinity Tryptophan Uptake into Human Cells33261077
POLRMT mutations impair mitochondrial transcription causing neurological disease33602924
Indole-linked 1, 2, 3-triazole derivatives efficiently modulate COX-2 protein levels in human THP-1 monocytes by suppressing AGE-ROS-NF-kβ nexusPubmed:34990649
Isolation and characterization of anti-inflammatory and anti-proliferative compound, for B-cell Non-Hodgkin lymphoma, from Nyctanthes arbor-tristis Linn.Pubmed:35398498
Investigation of single beam ultrasound sensitivity as a monitoring tool for local hyperthermia treatment in breast cancer