Anti-Beta Actin (ACTB) Polyclonal Antibody Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Ovis aries; Ovine (Sheep), Equus caballus; Equine (Horse), Chicken (Gallus) Control antibody

Actin Beta; ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed

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Overview
Properties
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.1-1mg/ml
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  • Anti-Beta Actin (ACTB) Polyclonal Antibody Packages (Simulation)
  • Anti-Beta Actin (ACTB) Polyclonal Antibody Packages (Simulation)
  • CAB340Mi01.png Figure. Western Blot; Sample: Recombinant ACTb.
  • Anti-Beta Actin (ACTB) Polyclonal Antibody Figure . Western Blot
    Lane1: Human Liver Tissue
    Lane2: Human Lung Tissue
    Lane3: Human K562 Cells
    Lane4: Mouse Lung Tissue
    Primary Ab: 1:500  Dilution of Rabbit Anti-Human ACTb Ab
    Second Ab: 1:2000 Dilution of HRP-Linked Guinea pig Anti-Rabbit Ab (Catalog: SAA544Rb59)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against ACTB. It has been selected for its ability to recognize ACTB in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;1:500-2000
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and L-Glutamine on Molecular Responses in Chicken Embryo Pectoral MusclesNCBI:PMC3856104
  • Expression of chloride channels in mouse skin incisional wounds96
  • Targeted Elimination of Senescent Beta Cells Prevents Type 1 Diabetes
  • Neuroinflammation, Oxidative Stress, and Neurogenesis in a Mouse Model of Chronic Fatigue Syndrome, and the Treatment with Kampo MedicinePubmed: 31902915
  • Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor …Pubmed: 32046779
  • Effect of ischemic postconditioning on cell apoptosis and expression of relevant genes in non-culprit coronary arteriesPubmed: 32784207
  • Malted Barley Improved the Structure and Function of Gastrocnemius Muscle of Hypercholesterolemic Mother Rats and their Offspring
  • Tryptophan Depletion Modulates Tryptophanyl-tRNA Synthetase-Mediated High-Affinity Tryptophan Uptake into Human Cells33261077
  • POLRMT mutations impair mitochondrial transcription causing neurological disease33602924
  • Indole-linked 1, 2, 3-triazole derivatives efficiently modulate COX-2 protein levels in human THP-1 monocytes by suppressing AGE-ROS-NF-kβ nexusPubmed:34990649
  • Isolation and characterization of anti-inflammatory and anti-proliferative compound, for B-cell Non-Hodgkin lymphoma, from Nyctanthes arbor-tristis Linn.Pubmed:35398498
  • Investigation of single beam ultrasound sensitivity as a monitoring tool for local hyperthermia treatment in breast cancer

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