Anti-Extracellular Signal Regulated Kinase 1 (ERK1) Polyclonal Antibody Homo sapiens (Human) Control antibody

HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; PRKM3; MAPK3; ERT2; Mitogen-Activated Protein Kinase 3; Insulin-stimulated MAP2 kinase; Microtubule-associated protein 2 kinase

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Overview
Properties
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Anti-Extracellular Signal Regulated Kinase 1 (ERK1) Polyclonal Antibody Packages (Simulation)
  • Anti-Extracellular Signal Regulated Kinase 1 (ERK1) Polyclonal Antibody Packages (Simulation)
  • Anti-Extracellular Signal Regulated Kinase 1 (ERK1) Polyclonal Antibody DAB staining on IHC-P; Sample: Porcine Cerebrum Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human ERK1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Anti-Extracellular Signal Regulated Kinase 1 (ERK1) Polyclonal Antibody DAB staining on IHC-P;
    Sample: Porcine Kidney Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Human ERK1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Anti-Extracellular Signal Regulated Kinase 1 (ERK1) Polyclonal Antibody DAB staining on IHC-P;
    Sample: Porcine Liver Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Human ERK1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Anti-Extracellular Signal Regulated Kinase 1 (ERK1) Polyclonal Antibody DAB staining on IHC-P;
    Sample: Human Ovarian cancer Tissue;
    Primary Ab: 10ug/ml Rabbit Anti-Human ERK1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Anti-Extracellular Signal Regulated Kinase 1 (ERK1) Polyclonal Antibody DAB staining on IHC-P;
    Sample: Human Colorectal cancer Tissue;
    Primary Ab: 10ug/ml Rabbit Anti-Human ERK1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Anti-Extracellular Signal Regulated Kinase 1 (ERK1) Polyclonal Antibody DAB staining on IHC-P;
    Sample: Human Lung cancer Tissue;
    Primary Ab: 10ug/ml Rabbit Anti-Human ERK1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Anti-Extracellular Signal Regulated Kinase 1 (ERK1) Polyclonal Antibody DAB staining on IHC-P;
    Sample: Human Ovarian cancer Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human ERK1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Anti-Extracellular Signal Regulated Kinase 1 (ERK1) Polyclonal Antibody DAB staining on IHC-P;
    Sample: Human Colorectal cancer Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human ERK1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Anti-Extracellular Signal Regulated Kinase 1 (ERK1) Polyclonal Antibody DAB staining on IHC-P;
    Sample: Human Lung cancer Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human ERK1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Anti-Extracellular Signal Regulated Kinase 1 (ERK1) Polyclonal Antibody FITC staining on IF;
    Sample: Hela cell
    Primary Ab: 20µg/ml Rabbit Anti-Human ERK1 Antibody
    Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • CAB357Hu01.jpg Western Blot; Sample: Lane1: MCF7 cell lysate; Lane2: 293T cell lysate Primary Ab: 0.2µg/ml Rabbit Anti-Human ERK1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Anti-Extracellular Signal Regulated Kinase 1 (ERK1) Polyclonal Antibody Western Blot; Sample: Lane1: Rat Liver lysate; Lane2: Rat Cerebrum lysate; Lane3: Mouse Liver lysate; Lane4: Mouse Cerebrum lysate; Lane5: Mouse Heart lysate; Lane6: Porcine Liver lysate; Lane7: Porcine Cerebrum lysate
    Primary Ab: 0.2µg/ml Rabbit Anti-Human ERK1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Anti-Extracellular Signal Regulated Kinase 1 (ERK1) Polyclonal Antibody Western Blot; Sample: Lane1: Caprine Liver lysate; Lane2: Caprine Cerebrum lysate; Lane3: Caprine Heart lysate; Lane4: Canine Cerebrum lysate; Lane5: Gallus Cerebrum lysate; Lane6: Cavia Cerebrum lysate; Lane7: Equine Heart lysate
    Primary Ab: 0.2µg/ml Rabbit Anti-Human ERK1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against ERK1. It has been selected for its ability to recognize ERK1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2ug/ml
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndromepubmed:27538909
  • Gonadotropin Stimulation Has Only a Limited Effect on the Concentration of Follicular Fluid Signalling Proteins: An Antibody Array Analysis33575317
  • Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells34837026

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