Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Monoclonal Antibody

G3PD; GAPD; Peptidyl-cysteine S-nitrosylase GAPDH

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 119.00 US$ 279.00 US$ 398.00 US$ 995.00 US$ 3,980.00
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Overview

Properties

Product No.CAB932Hu22
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsLoading Control of WB;IHC;IF
Research use only
DownloadInstruction Manual
CategoryEnzyme & Kinase Metabolic pathway Cardiovascular biology Hepatology Nutrition metabolism

PurificationProtein A + Protein G affinity chromatography
LabelNone
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 1mg/mL

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 10ug/ml Mouse Anti-Human GAPDH Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Colorectal cancer Tissue;
Primary Ab: 10ug/ml Mouse Anti-Human GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Rat Ovary Tissue;
Primary Ab: 5ug/ml Mouse Anti-Human GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Rat Pancreas Tissue;
Primary Ab: 5ug/ml Mouse Anti-Human GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Mouse Skeletal muscle Tissue;
Primary Ab: 5ug/ml Mouse Anti-Human GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Mouse Cerebrum Tissue;
Primary Ab: 5ug/ml Mouse Anti-Human GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
FITC staining on IF;
Sample: Human HepG2 cell;
Primary Ab: 20μg/ml Mouse Anti-Human GAPDH Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18)
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 30µg/ml Mouse Anti-Human GAPDH Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)

Western Blot; Sample: Human Lung lysate; Primary Ab: 5µg/ml Mouse Anti-Human GAPDH Antibody Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
Western Blot; Sample: Human A549 cell lysate;
Primary Ab: 5µg/ml Mouse Anti-Human GAPDH Antibody
Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot; Sample: Human 293T cell lysate;
Primary Ab: 5µg/ml Mouse Anti-Human GAPDH Antibody
Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 5µg/ml Mouse Anti-Human GAPDH Antibody
Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot Sample: Lane1: Human Lung lysate; Lane2: 293T cell lysate; Lane3: Cavia Kidney lysate; Lane4: Bovine Liver lysate; Lane5: Bovine Kidney lysate; Lane6: Caprine Liver lysate; Lane7: Mouse Liver lysate; Lane8: Rat Liver lysate; Lane9: Rat Kidney lysate;
Primary Ab: 1μg/ml Mouse Anti-Human GAPDH Antibody
Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against GAPDH. It has been selected for its ability to recognize GAPDH in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.1ng/ml-100ng/ml;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence:5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Cavia (Guinea pig ), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Ovis aries; Ovine (Sheep)

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Citations

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