Anti-Heat Shock Protein 60 (Hsp60) Polyclonal Antibody

HSPD1; GROEL; CPN60; HuCHA60; Heat Shock 60kD Protein 1, Chaperonin; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein

UOM
1. 25test
2. 50test
3. 100test
4. 200test
5. 500test
FOB US$ 120.00 US$ 200.00 US$ 320.00 US$ 400.00 US$ 700.00
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Overview

Properties

Product No.FAA822Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsFlow cytometry.
Research use only
Downloadn/a
CategorySignal transduction Tumor immunity

Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Excitation LaserNone
Vol. per test1-5 µl
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid

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Packages (Simulation)
Packages (Simulation)

HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-human HSPD1 Polyclonal Antibody (Catalog # FAA822Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.

ISO9001: 2008, ISO13485: 2003 Registered

Usage

For flow cytometry, the suggested use of this reagent is 1-5 µl per 10^6 cells in 100 µl volume.
Optimal working dilutions could be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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Citations

Hsp60 response in experimental and human temporal lobe epilepsyPubMed: 25801186
Senescent hepatocytes in decompensated liver show reduced UPR and its key player, CLPP, attenuates senescence in vitroPubmed: 30878663
Activation of mitochondrial unfolded protein response is associated with Her2-overexpression breast cancerPubmed: 32601970