Anti-Heat Shock Protein 60 (Hsp60) Monoclonal Antibody Homo sapiens (Human) Protein, Antibody, Assaykit, Service

HSPD1; GROEL; CPN60; HuCHA60; Heat Shock 60kD Protein 1, Chaperonin; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein

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Overview
Properties
  • Ig Isotype IgG, Clone Number D1
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Excitation Laser
  • Vol. per test
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
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  • Anti-Heat Shock Protein 60 (Hsp60) Monoclonal Antibody Packages (Simulation)
  • Anti-Heat Shock Protein 60 (Hsp60) Monoclonal Antibody Packages (Simulation)
  • Anti-Heat Shock Protein 60 (Hsp60) Monoclonal Antibody Human Hela cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human Hsp60 Monoclonal Antibody (Catalog FAA822Hu02, red histogram) or Isotype control antibody (Catalog IS067-Mu01, green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Usage

Western blotting: 0.2-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:10μg/ml;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Giveaways

Citations

  • Hsp60 response in experimental and human temporal lobe epilepsyPubMed: 25801186
  • Senescent hepatocytes in decompensated liver show reduced UPR and its key player, CLPP, attenuates senescence in vitroPubmed: 30878663
  • Activation of mitochondrial unfolded protein response is associated with Her2-overexpression breast cancerPubmed: 32601970

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