Anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Polyclonal Antibody

EAT; MCL1L; MCL1S; BCL2L3; TM; Myeloid Cell Leukemia Sequence 1, Bcl2 Related; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; mcl1/EAT

UOM
1. 25test
2. 50test
3. 100test
4. 200test
5. 500test
FOB US$ 132.00 US$ 220.00 US$ 352.00 US$ 440.00 US$ 770.00
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Overview

Properties

Product No.FAC615Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsFlow cytometry.
Research use only
Downloadn/a
CategoryTumor immunity

Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Excitation LaserNone
Vol. per test1-5 µl
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid

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Packages (Simulation)
Packages (Simulation)

Human Raji cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human MCL1 Polyclonal Antibody (Catalog FAC615Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

ISO9001: 2008, ISO13485: 2003 Registered

Usage

For flow cytometry, the suggested use of this reagent is 1-5 µl per 10^6 cells in 100 µl volume.
Optimal working dilutions could be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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Citations

Rationale for a Combination Therapy Consisting of MCL1-and MEK-Inhibitors in Acute Myeloid LeukemiaPubmed: 31718075
Restoration of miR-193a expression is tumor-suppressive in MYC amplified Group 3 medulloblastomaPubmed: 32410663
BMI1-Inhibitor PTC596 in Combination with MCL1 Inhibitor S63845 or MEK Inhibitor Trametinib in the Treatment of Acute Leukemia. Cancers 2021, 13, 58133540760