Anti-Proliferating Cell Nuclear Antigen (PCNA) Monoclonal Antibody Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Canis familiaris; Canine (Dog) Control antibody

Cyclin

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Overview
Properties
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Anti-Proliferating Cell Nuclear Antigen (PCNA) Monoclonal Antibody Packages (Simulation)
  • Anti-Proliferating Cell Nuclear Antigen (PCNA) Monoclonal Antibody Packages (Simulation)
  • CAA591Mi22.jpg WB Image
  • Anti-Proliferating Cell Nuclear Antigen (PCNA) Monoclonal Antibody Western Blot; Sample: Lane1: A549 cell lysate; Lane2: 293T cell lysate; Lane3: A875 cell lysate; Lane4: HepG2 cell lysate; Lane5: Hela cell lysate; Lane6: K562 cell lysate; Lane7: MCF7 cell lysate
    Primary Ab: 0.5µg/ml Mouse Anti-Multi-species PCNA Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Anti-Proliferating Cell Nuclear Antigen (PCNA) Monoclonal Antibody Western Blot; Sample: Lane1: U87MG cell lysate; Lane2: SGC7901 cell lysate; Lane3: ECV304 cell lysate; Lane4: PC3 cell lysate; Lane5: A375 cell lysate; Lane6: A431 cell lysate; Lane7: Jurkat cell lysate
    Primary Ab: 0.5µg/ml Mouse Anti-Multi-species PCNA Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Anti-Proliferating Cell Nuclear Antigen (PCNA) Monoclonal Antibody Western Blot; Sample: Lane1: Mouse Testis lysate; Lane2: Mouse Small intestine lysate; Lane3: Rat Spleen lysate; Lane4: Porcine Heart lysate; Lane5: Porcine Liver lysate; Lane6: Porcine Spleen lysate; Lane7: Bovine Cerebrum lysate; Lane8: Bovine Heart lysate; Lane9: Canine Liver lysate
    Primary Ab: 0.5µg/ml Mouse Anti-Multi-species PCNA Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Anti-Proliferating Cell Nuclear Antigen (PCNA) Monoclonal Antibody Western Blot; Sample: Lane1: Cavia Heart lysate; Lane2: Cavia Liver lysate; Lane3: Cavia Spleen lysate; Lane4: Caprine Cerebrum lysate; Lane5: Caprine Heart lysate; Lane6: Caprine Liver lysate; Lane7: Gallus Heart lysate
    Primary Ab: 0.5µg/ml Mouse Anti-Multi-species PCNA Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against PCNA. It has been selected for its ability to recognize PCNA in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.0003-0.1ug/ml;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Hamster (Chinese hamster), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Ovis aries; Ovine (Sheep), Chicken (Gallus)

Giveaways

Citations

  • Human umbilical cord blood-mesenchymal stem cells transplantation renovates the ovarian surface epithelium in a rat model of premature ovarian failure: Possible direct and indirect effectsPubmed:27233913
  • Antioxidant, anti-inflammatory and synergistic anti-hyperglycemic effects of Malaysian propolis and metformin in streptozotocin–induced diabetic ratsPubmed:30026088
  • Malaysian propolis, metformin and their combination, exert hepatoprotective effect in streptozotocin-induced diabetic ratsPubmed: 30205096
  • Diabetes-induced testicular oxidative stress, inflammation, and caspase-dependent apoptosis: the protective role of metforminPubmed: 30513216
  • Synthesis of novel S-linked dihydroartemisinin derivatives and evaluation of their anticancer activityPubmed: 31216504
  • The Effect of Dietary Quercetin on the Glutathione Redox System and Small Intestinal Functionality of Weaned PigletsPubmed: 31426309
  • Oxidative Stress, NF-κB-Mediated Inflammation and Apoptosis in the Testes of Streptozotocin–Induced Diabetic Rats: Combined Protective Effects of Malaysian …Pubmed: 31600920
  • Tert-butylhydroquinone preserve testicular steroidogenesis and spermatogenesis in cisplatin-intoxicated rats by targeting oxidative stress, inflammation and apoptosisPubmed: 32565124
  • Protective effects of bee bread on testicular oxidative stress, NF-κB-mediated inflammation, apoptosis and lactate transport decline in obese male ratsPubmed: 33152939
  • Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats33750916
  • Local DNA synthesis is critical for DNA repair during oocyte maturation34415018
  • Comparative Evaluation of Anti-Fibrotic Effect of Tissue Specific Mesenchymal Stem Cells Derived Extracellular Vesicles for the Amelioration of CCl4 Induced Chronic …34859376
  • Inflammatory Modulation of miR-155 Inhibits Doxorubicin‑Induced Testicular Dysfunction Via Sirt1/Foxo1 Pathway: Insight to the Role of Acacetin and Bacillus …Pubmed:35715546
  • Therapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model …Pubmed:35204140
  • Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β …
  • Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation …

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