Anti-Succinate Dehydrogenase Complex Subunit A (SDHA) Polyclonal Antibody Mus musculus (Mouse) Control antibody

SDH2; SDHF; FP; Flavoprotein; Flavoprotein subunit of complex II; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

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Overview
Properties
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Anti-Succinate Dehydrogenase Complex Subunit A (SDHA) Polyclonal Antibody Packages (Simulation)
  • Anti-Succinate Dehydrogenase Complex Subunit A (SDHA) Polyclonal Antibody Packages (Simulation)
  • CAJ784Mi01.jpg Western Blot; Sample: Lane1: Mouse Heart lysate; Lane2: Mouse Liver lysate; Lane3: Mouse Cerebrum lysate; Lane4: Mouse Kidney lysate Primary Ab: 1µg/ml Rabbit Anti-Multi-species SDHA Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against SDHA. It has been selected for its ability to recognize SDHA in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Macrophage-derived extracellular succinate licenses neural stem cells to suppress chronic neuroinflammationPubmed:29478844

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