Anti-Tubulin Beta (TUBb) Polyclonal Antibody 
TUB-B; TUBB5; TUBB1; M40
Overview
Properties
- Product No.CAB870Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsLoading Control of WB.
Research use only - DownloadInstruction Manual
- CategoryNeuro scienceDevelopmental science
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 500µg/mL
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Packages (Simulation)
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Packages (Simulation)
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Figure. Western Blot; Sample: Recombinant TUBb, Human.
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Figure. Western Blot; Sample: Rat Liver Tissue; Lane2: Rat Kidney Tissue; Lane3: Mouse Liver Tissue; Lane4: Mouse Kidney Tissue; Lane5: Mouse Brain Tissue.
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Western Blot; Samples: Lane1: Rat Cerebrum lysate; Lane2: Mouse Cerebrum lysate; Lane3: Porcine Cerebrum lysate; Lane4: Bovine Cerebrum lysate;
Primary Ab: 0.1µg/ml Rabbit Anti-Human TUBb Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The antibody is a rabbit polyclonal antibody raised against TUBb. It has been selected for its ability to recognize TUBb in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.5-2ug/ml
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Cavia (Guinea pig ), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Chicken (Gallus), Danio rerio (Zebrafish)Giveaways
Increment services
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
Citations
- Synthesis and cytotoxic activity of certain trisubstituted azetidin-2-one derivatives as a cis-restricted combretastatin A-4 analoguespubmed:27747473
- New 3-Substituted-2-(4-hydroxyanilino)pyridine Derivatives: Synthesis, Antitumor Activity, and Tubulin Polymerization Inhibition.pubmed:28150327
- Novel Beta-Tubulin-Immobilized Nanoparticles Affinity Material for Screening β-Tubulin Inhibitors from a Complex Mixturepubmed:28112513
- Design, synthesis and biological evaluation of chromenopyrimidines as potential cytotoxic agentsPubmed:29779400
- Design and synthesis of novel 5-(4-chlorophenyl) furan derivatives with inhibitory activity on tubulin polymerizationPubmed:29966433
- Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipidsPubmed: 30237167
- Design, synthesis, and screening of amino thiophene carboxamide derivatives on hepatocellular carcinomaas VEGFR-2InhibitorsPubmed: 30191744
- Potent combretastatin A-4 analogs containing 1, 2, 4-triazole: Synthesis, antiproliferative, anti-tubulin activity, and docking studyPubmed: 31536891
- Early diagnosis of Alzheimer's disease in blood using a disposable electrochemical microfluidic platformPubmed: 32207606
- Tubulin Inhibitors: Discovery of a New Scaffold Targeting Extra-binding Residues within the Colchicine Site through Anchoring Substituents Properly Adapted to their …Pubmed: 32325332
- Ginger and turmeric lipid-solubles attenuate heated oil-induced cardio-hepatic oxidative stress via the upregulation of NRF2 and decrease blood pressure in ratsPubmed: 33028437
- Design, Synthesis and Screening of Benzimidazole Containing Compounds with Methoxylated Aryl Radicals as Cytotoxic Molecules on (HCT-116) Colon …Pubmed: 33158579
- Cytotoxicity, in silico predictions and molecular studies for androstane heterocycle compounds revealed potential antitumor agent against lung cancer cells33300466
- Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins
- Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression34376212
- Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells34837026