Anti-Vinculin (VCL) Monoclonal Antibody Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat) Control antibody

MVCL; Metavinculin

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Overview
Properties
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Anti-Vinculin (VCL) Monoclonal Antibody Packages (Simulation)
  • Anti-Vinculin (VCL) Monoclonal Antibody Packages (Simulation)
  • CAB839Mi22.jpg Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Porcine Heart lysate; Lane3: Porcine Skeletal muscle lysate Primary Ab: 3µg/ml Mouse Anti-Multi-species VCL Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Anti-Vinculin (VCL) Monoclonal Antibody Western Blot; Sample: Lane1: Mouse Lung lysate; Lane2: Rat Lung lysate; Lane3: Canine Heart lysate; Lane4: Gallus Heart lysate; Lane5: Bovine Heart lysate; Lane6: Rabbit Lung lysate; Lane7: Rabbit Heart lysate; Lane8: Cavia Lung lysate; Lane9: Cavia Heart lysate
    Primary Ab: 2µg/ml Mouse Anti-Multi-species VCL Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Anti-Vinculin (VCL) Monoclonal Antibody Western Blot; Sample: Lane1: Mouse Heart lysate; Lane2: Rat Lung lysate; Lane3: Canine Heart lysate; Lane4: Gallus Heart lysate; Lane5: Bovine Heart lysate; Lane6: Rabbit Lung lysate; Lane7: Cavia Lung lysate
    Primary Ab: 2μg/ml Mouse Anti-Multi-species VCL Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against VCL. It has been selected for its ability to recognize VCL in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.1-1µg/ml;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Cavia (Guinea pig ), Oryctolagus cuniculus (Rabbit), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Chicken (Gallus)

Giveaways

Citations

  • S-Nitrosylation Proteome Profile of Peripheral Blood Mononuclear Cells in Human Heart Failurejournals:1384523
  • PCTAIRE1 promotes mitotic progression and resistance against antimitotic and apoptotic signalsPubmed:35044463

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