Polyclonal Antibody to Angiotensin I Converting Enzyme (ACE) Homo sapiens (Human) Polyclonal antibody

CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA004Hu01-Recombinant Angiotensin I Converting Enzyme (ACE)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Angiotensin I Converting Enzyme (ACE) Packages (Simulation)
  • Polyclonal Antibody to Angiotensin I Converting Enzyme (ACE) Packages (Simulation)
  • Polyclonal Antibody to Angiotensin I Converting Enzyme (ACE) Figure. DAB staining on IHC-P; Samples: Human Kidney Tissue.
  • Polyclonal Antibody to Angiotensin I Converting Enzyme (ACE) Figure. HE staining on IHC-P;
    Samples: Human Intestine Tissue.
  • PAA004Hu01.jpg Figure. Western Blot; Sample: Recombinant ACE, Human.
  • Polyclonal Antibody to Angiotensin I Converting Enzyme (ACE) Figure. Western Blot; Sample: Human Lung Tissue.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against ACE. It has been selected for its ability to recognize ACE in immunohistochemical staining and western blotting.

Usage

Western blotting: 1-5µg/mL
Immunocytochemistry in formalin fixed cells: 5-20µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
Immunohistochemistry in paraffin section: 5-20µg/mL
Enzyme-linked Immunosorbent Assay: 0.05-2µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Association of elevated blood pressure and impaired vasorelaxation in experimental Sprague-Dawley rats fed with heated vegetable oilPubMed: PMC2914008
  • Effect of Repeatedly Heated Palm Olein on Blood Pressure–Regulating Enzymes Activity and Lipid Peroxidation in RatsUsm: MJMS19120
  • Plasma Levels of Angiotensin-Converting Enzymes 1 and 2 and AGTR2 (T1247G and A5235G) Gene Polymorphisms Are Associated to Breast Cancer Progression.Scirp: Source
  • Vascular function and atherosclerosis progression after 1 y of flavonoid intake in statin-treated postmenopausal women with type 2 diabetes: a double-blind randomized controlled trial.Pubmed: 23553151
  • Cathepsin G deficiency reduces periaortic calcium chloride injury-induced abdominal aortic aneurysms in mice Pubmed:25037606
  • ACE2 activation by xanthenone prevents leptin-induced increases in blood pressure and proteinuria during pregnancy in Sprague-Dawley ratsPubmed:25205467
  • Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal InstillationPubmed:25580880
  • Arsenic causes aortic dysfunction and systemic hypertension in rats: Augmentation of angiotensin II signalingPubMed: 26079204
  • Protective Effect of Enalapril against Methionine-Enriched Diet-Induced Hypertension: Role of Endoplasmic Reticulum and Oxidative StressPubMed: 26640794
  • MechanismsPubMed: 26602523
  • Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal InstillationPubMed: 25580880
  • Angiotensin converting enzymes in patients with acute respiratory distress syndromeContent: 3
  • Garlic Attenuates Plasma and Kidney ACE-1 and AngII Modulations in Early Streptozotocin-Induced Diabetic Rats: Renal Clearance and Blood Pressure ImplicationsPubmed:27293465
  • Ренин-ангиотензин-альдостероновая система у крыс линии НИСАГ (ISIAH) со стресс-индуцированной артериальной гипертензией30_Dubinina.pdf
  • The effect of heat stress on bull sperm quality and related HSPs expressioncontent:journals
  • Citrus leaf extract reduces blood pressure and vascular damage in repeatedly heated palm oil diet-Induced hypertensive rats.pubmed:28068636

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