Polyclonal Antibody to Angiotensinogen (AGT) Rattus norvegicus (Rat) Polyclonal antibody

ANHU; SERPINA8; Angiotensin; Renin Substrate; Serpin Peptidase Inhibitor,Clade A,Member 8(Alpha-1 Antiproteinase,Antitrypsin)

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA797Ra01-Recombinant Angiotensinogen (AGT)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Angiotensinogen (AGT) Packages (Simulation)
  • Polyclonal Antibody to Angiotensinogen (AGT) Packages (Simulation)
  • Polyclonal Antibody to Angiotensinogen (AGT) IHC Images
  • PAA797Ra01.jpg Figure. Western Blot; Sample: Recombinant AGT, Rat.
  • Polyclonal Antibody to Angiotensinogen (AGT) Western Blot; Sample: Rat Serum;
    Primary Ab: 1µg/ml Rabbit Anti-Rat AGT Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Angiotensinogen (AGT) Western Blot; Sample: Rat Kidney lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Rat AGT Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Angiotensinogen (AGT) Western Blot; Sample: Rat Liver lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Rat AGT Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against AGT. It has been selected for its ability to recognize AGT in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;1:500-2000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Elevated urinary angiotensinogen a marker of intrarenal renin angiotensin system in hypertensive renal transplant recipients: does it play a role in development of proteinuria in hypertensive renal transplant patients?Wiley: source
  • The relation between urinary angiotensinogen and proteinuria in renal AA amyloidosis patientsPubMed: 22320202
  • Urinary angiotensinogen as a marker of intrarenal angiotensin II activity in adolescents with primary hypertensionPubMed: PMC3661928
  • Urinary angiotensinogen as a novel marker of obstructive nephropathy in childrenPubmed: 23772991
  • Association between urinary angiotensinogen, hypertension and proteinuria in pregnant women with preeclampsiaSagepub: Source
  • MP707 CHARACTERISTICS AND OUTCOMES OF CHILDREN WITH AUTOSOMAL RECESSIVE POLYCYSTIC KIDNEY DISEASE COHORT FROM THE ESPN/ERA-EDTA REGISTRYOxfordjournals:Source
  • Relationship between blood pressure varPubMed: 26223347
  • Higher Serum Angiotensinogen Is an Indicator of IgA Vasculitis with Nephritis Revealed by Comparative Proteomes AnalysisPubMed: 26098644
  • Korelasi pengukuran antropometri dengan tekanan darah dan angiotensinogen plasma pada dewasaIndex.Php: Art
  • Relationship between blood pressure variability and renal activity of the renin-angiotensin system.pubmed:26223347
  • Pharmacological evidence: a new therapeutic approach to the treatment of chronic heart failure through SUR2B/Kir6.1 channel in endothelial cells.pubmed:27890915
  • Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosisS0021967317311858
  • Pyrrolidine dithiocarbamate inhibits mouse acute kidney injury induced by diclofenac by targeting oxidative damage, cytokines and NF-κB activityPubmed:30036488
  • Role of (Pro) Renin Receptor in Albumin Overload-Induced Nephropathy in RatsPubmed:29846109
  • Quantitation of Total and Free Thiol Angiotensinogen as a Prognostic Marker for PreeclampsiaPubmed: 31069778
  • Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans33407555
  • Association of Ang-(1¨C7) and des-Arg 9 BK as new biomarkers of obesity and cardiometabolic risk factors in adolescents33568792
  • (Pro) renin receptor antagonist PRO20 attenuates nephrectomy©\induced nephropathy in rats via inhibition of intrarenal RAS and Wnt/¦Â©\catenin signaling34057312
  • A novel strategy to identify candidate diagnostic and prognostic biomarkers for gastric cancer34215253

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