Polyclonal Antibody to Anterior Gradient 2 (AGR2) Mus musculus (Mouse) Polyclonal antibody

GOB4; HAG2; XAG2; AG2; PDIA17; HPC8; Anterior Gradient Protein 2; Protein Disulfide Isomerase Family A,Member 17; Secreted cement gland protein XAG-2 homolog

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPC285Mu01-Recombinant Anterior Gradient 2 (AGR2)
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Anterior Gradient 2 (AGR2) Packages (Simulation)
  • Polyclonal Antibody to Anterior Gradient 2 (AGR2) Packages (Simulation)
  • Polyclonal Antibody to Anterior Gradient 2 (AGR2) DAB staining on IHC-P; Samples: Mouse Stomach Tissue; Primary Ab: 10µg/ml Rabbit Anti-Mouse AGR2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Anterior Gradient 2 (AGR2) DAB staining on IHC-P;
    Samples: Mouse Stomach Tissue
  • Polyclonal Antibody to Anterior Gradient 2 (AGR2) DAB staining on IHC-P;
    Sample: Mouse Stomach Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Mouse AGR2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAC285Mu01.jpg WB Image
  • Polyclonal Antibody to Anterior Gradient 2 (AGR2) Western Blot; Sample: Lane1: Mouse Stomach lysate; Lane2: Mouse Testis lysate; Lane3: Rat Small intestine lysate
    Primary Ab: 2µg/mL Rabbit Anti-Mouse AGR2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Anterior Gradient 2 (AGR2) Western Blot; Sample: Mouse Stomach lysate;
    Primary Ab: 5µg/ml Rabbit Anti-Mouse AGR2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Anterior Gradient 2 (AGR2) Western Blot; Sample: Mouse Testis lysate;
    Primary Ab: 5µg/ml Rabbit Anti-Mouse AGR2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Anterior Gradient 2 (AGR2) Western Blot; Sample: Rat Small intestine lysate;
    Primary Ab: 5µg/ml Rabbit Anti-Mouse AGR2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against AGR2. It has been selected for its ability to recognize AGR2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Rattus norvegicus (Rat)

Giveaways

Citations

  • Serum AGR2 as an early diagnostic and postoperative prognostic biomarker of human lung adenocarcinomaMetaPress: n7137r6271u54118
  • Integrated Proteomic Profiling of Cell Line Conditioned Media and Pancreatic Juice for the Identification of Pancreatic Cancer BiomarkersPubMed: PMC3205865
  • Anterior gradient 2 (AGR2): blood-based biomarker elevated in metastatic prostate cancer associated with the neuroendocrine phenotypePubmed: 22911164
  • Validation of four candidate pancreatic cancer serological biomarkers that improve the performance of CA19.9Pubmed: 24007603
  • Validation of Biomarkers That Complement CA19.9 in Detecting Early Pancreatic CancerPubmed:25239611
  • AGR3 in Breast Cancer: Prognostic Impact and Suitable Serum-Based Biomarker for Early Cancer DetectionPubMed: 25875093
  • Serum AGR2 as a useful biomarker for pituitary adenomasS0303846717300045
  • Identification, characterization and application of a new peptide against anterior gradient homolog 2 (AGR2)Pubmed:29937991
  • Anterior gradient protein 2 is a marker of tumor aggressiveness in breast cancer and favors chemotherapy‑induced senescence escape34913074
  • Proteome profiling of enzalutamide‐resistant cell lines and serum analysis identified ALCAM as marker of resistance in castration‐resistant prostate cancerPubmed:35689436

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