Polyclonal Antibody to Apoptosis Inducing Factor (AIF) Rattus norvegicus (Rat) Polyclonal antibody

PDCD8; Programmed Cell Death protein 8; Apoptosis-inducing factor 1, mitochondrial

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB064Ra01-Recombinant Apoptosis Inducing Factor (AIF)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/mL
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  • Polyclonal Antibody to Apoptosis Inducing Factor (AIF) Packages (Simulation)
  • Polyclonal Antibody to Apoptosis Inducing Factor (AIF) Packages (Simulation)
  • Polyclonal Antibody to Apoptosis Inducing Factor (AIF) DAB staining on IHC-P;
    Sample: Rat Liver Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Rat AIF Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Apoptosis Inducing Factor (AIF) Western Blot; Samples: Lane1: Hela cell lysate; Lane2: K562 cell lysate; Lane3: Jurkat cell lysate;
    Primary Ab: 0.1µg/ml Rabbit Anti-Rat AIF Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against AIF. It has been selected for its ability to recognize AIF in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human)

Giveaways

Citations

  • РОЛЬ АПОПТОЗ ИНДУЦИРУЮЩЕГО ФАКТОРА И ОКИСЛИТЕЛЬНОГО СТРЕССА ПРИ ХРОНИЧЕСКОЙ СЕРДЕЧНОЙ НЕДОСТАТОЧНОСТИ616-005.8
  • Redox-active DJ-1 sustains brainstem cardiovascular regulation via maintenance of mitochondrial function during mevinphos intoxicationPubmed: 32650030
  • Chromon-3-aldehyde derivatives restore mitochondrial function in rat cerebral ischemiaPubmed: 32963739
  • Correction of mitochondrial dysfunction by 4-hydroxy-3, 5-ditretbutyl cinnamic acid in experimental Alzheimer's disease induced by A¦Â injection in rats.

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