Monoclonal Antibody to Apoptosis Signal Regulating Kinase 1 (ASK1)
MAP3-K5; MAP3K5; ASK1; MAPKKK5; MEKK5; Mitogen-Activated Protein Kinase Kinase Kinase 5; MAPK/ERK kinase kinase 5
Overview
Properties
- Product No.MAB358Hu22
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - Downloadn/a
- CategoryEnzyme & KinaseApoptosis
- SourceMonoclonal antibody preparation, Host Mouse
- Ig Isotype IgG, Clone Number n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 1mg/mL
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Specifity
The antibody is a mouse monoclonal antibody raised against ASK1. It has been selected for its ability to recognize ASK1 in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
Citations
- Heat-shock protein 70 modulates apoptosis signal-regulating kinase 1 in stressed hepatocytes of Mugil cephalusPubmed:24875452
- Differential reCavia (Guinea pig )lation of pro- and antiapoptotic proteins in fish adipocytes during hypoxic conditions.Pubmed:26744268
- ASK-1 Expression Down-regulated in Preeclamptic Placental Explants Incubated with Mint-Tea:
- Differential expression of survival proteins during decreased intracellular oxygen tension in brain endothelial cells of grey mullets.pubmed:28043644
- Upregulation of HSP70 Extends Cytoprotection to Fish Brain under Xenobiotic Stressb0407ebde042c4f61b728bc11a586e6c