Monoclonal Antibody to Arginase (ARG) Homo sapiens (Human) Monoclonal antibody

ARG1; Arginase I; Liver Arginase

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG1 Kappa, Clone Number C5
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPB120Hu01-Recombinant Arginase (ARG)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Arginase (ARG) Packages (Simulation)
  • Monoclonal Antibody to Arginase (ARG) Packages (Simulation)
  • Monoclonal Antibody to Arginase (ARG) Vector Red staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 10µg/ml Mouse Anti-Human Arg Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Monoclonal Antibody
  • Monoclonal Antibody to Arginase (ARG) DAB staining on IHC-P;
    Sample: Human Liver Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Human Arg Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
    Secondary antibody only control: Used PBS instead of primary antibody , Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Arginase (ARG) DAB staining on IHC-P;
    Sample: Human Liver cancer Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Human Arg Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
    Secondary antibody only control: Used PBS instead of primary antibody , Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Arginase (ARG) DAB staining on IHC-P;
    Sample: Human Liver Tissue
    Primary Ab: 10µg/ml Mouse Anti-Human Arg Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Arginase (ARG) DAB staining on IHC-P;
    Sample: Human Liver cancer Tissue
    Primary Ab: 10µg/ml Mouse Anti-Human Arg Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Arginase (ARG) DAB staining on IHC-P;
    Sample: Porcine Liver Tissue
    Primary Ab: 30µg/ml Mouse Anti-Human Arg Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • MAB120Hu22.jpg Figure. Western Blot; Sample: Recombinant ARG, Human.
  • Monoclonal Antibody to Arginase (ARG) Western Blot; Sample: HepG2 cell lysate
    Primary Ab: 2µg/ml Mouse Anti-Human ARG Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19) Selected
  • Monoclonal Antibody to Arginase (ARG) Western Blot; Sample: Mouse Liver lysate
    Primary Ab: 2µg/ml Mouse Anti-Human ARG Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19) Selected
  • Monoclonal Antibody to Arginase (ARG) Western Blot; Sample: Lane1: Rat Liver lysate; Lane2: Porcine Liver lysate
    Primary Ab: 0.02µg/ml Mouse Anti-Human Arg Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19) Selected
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against ARG. It has been selected for its ability to recognize ARG in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • Phenobarbital Induces Alterations in the Proteome of Hepatocytes and Mesenchymal Cells of Rat LiversPubmed: 24204595
  • Arginase I release from activated neutrophils induces peripheral immunosuppression in a murine model of strokePubMed: 25966956
  • Circulating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host diseasePubmed:27109254
  • Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonicaPubmed:27157496
  • Potent but transient immunosuppression of T-cells is a general feature of erythroid progenitor cells
  • Potent but transient immunosuppression of T-cells is a general feature of CD71+ erythroid cells34893694

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