Monoclonal Antibody to Cluster Of Differentiation 86 (CD86) Homo sapiens (Human) Monoclonal antibody

LAB7-2; B7-2; B70; FUN-1; CD28LG2; CD28 Antigen Ligand 2,B7-2 Antigen; CTLA-4 counter-receptor B7.2; B-Lymphocyte Activation Antigen B7-2

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG1 Kappa, Clone Number C6
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPA824Hu01-Recombinant Cluster Of Differentiation 86 (CD86)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Cluster Of Differentiation 86 (CD86) Packages (Simulation)
  • Monoclonal Antibody to Cluster Of Differentiation 86 (CD86) Packages (Simulation)
  • Monoclonal Antibody to Cluster Of Differentiation 86 (CD86) AF488 staining on IF;
    Sample: MCF7 cell
    Primary Ab: 30µg/ml Mouse Anti-Human CD86 Antibody
    Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu11)
  • MAA824Hu22.jpg Western Blot; Sample: Jurkat cell lysate Primary Ab: 0.6µg/ml Mouse Anti-Human CD86 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against CD86. It has been selected for its ability to recognize CD86 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Toll-Like Receptor 2–Mediated Autophagy Promotes Microglial Cell Death by Modulating the Microglial M1/M2 PhenotypePubmed: 31834572
  • The phenotypical induction and damage effect of peptidoglycan on microglia

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