• KO-Validated
Polyclonal Antibody to Beclin 1 (BECN1) Homo sapiens (Human) Polyclonal antibody

ATG6; VPS30; Coiled-Coil,Myosin-Like BCL2 Interacting Protein; ATG6 Autophagy Related 6 Homolog

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPJ557Hu01-Recombinant Beclin 1 (BECN1)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/mL
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  • Polyclonal Antibody to Beclin 1 (BECN1) Packages (Simulation)
  • Polyclonal Antibody to Beclin 1 (BECN1) Packages (Simulation)
  • Polyclonal Antibody to Beclin 1 (BECN1) DAB staining on IHC-P; Samples: Human Cerebrum Tissue; Primary Ab: 30µg/ml Rabbit Anti-Human BECN1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Beclin 1 (BECN1) DAB staining on IHC-P;
    Samples: Human Pancreas Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Human BECN1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAJ557Hu01.jpg Western Blot; Sample: Rat Cerebrum lysate Primary Ab: 0.1µg/ml Rabbit Anti-Human BECN1 Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Beclin 1 (BECN1) Western Blot; Sample: Lane1: Porcine Cerebrum lysate; Lane2: Rat Cerebrum lysate; Lane3: Mouse Cerebrum lysate; Lane4: Canine Cerebrum lysate; Lane5: Bovine Cerebrum lysate; Lane6: Caprine Cerebrum lysate; Lane7: T-47D cell lysate
    Primary Ab: 0.3μg/ml Rabbit Anti-Human BECN1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19) Selected
  • Polyclonal Antibody to Beclin 1 (BECN1) Figure. Western Blot; Lane1: Human A431 Cells; Lane2: Human Hela Cells; Lane3: Porcine Brain Tissue.
  • Polyclonal Antibody to Beclin 1 (BECN1) Knockout Varification:
    Lane 1: Wild-type MCF7 cell lysate;
    Lane 2: BECN1 knockout MCF7 cell lysate;
    Predicted MW: 52kDa
    Observed MW: 60kDa
    Primary Ab: 3µg/ml Rabbit Anti-Human BECN1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Beclin 1 (BECN1) Western Blot; Samples: Lane1: Ovine Cerebrum lysate; Lane2: Bovine Cerebrum lysate; Lane3: MCF7 cell lysate; Lane4: A431 cell lysate;
    Primary Ab: 0.1µg/ml Rabbit Anti-Human BECN1 Antibody
    Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against BECN1. It has been selected for its ability to recognize BECN1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Ovis aries; Ovine (Sheep)

Giveaways

Citations

  • Can Enhanced Autophagy be Associated with Human Longevity? Serum Levels of the Autophagy Biomarker Beclin-1 are Increased in Healthy CentenariansPubmed:25307612
  • A peel-off facial mask comprising myoinositol and trehalose-loaded liposomes improves adult female acne by reducing local hyperandrogenism and activating autophagypubmed:28342238
  • The novel CXCR4 antagonist, PRX177561, reduces tumor cell proliferation and accelerates cancer stem cell differentiation in glioblastoma preclinical modelspubmed:28639900
  • Beclin1 circulating levels and accelerated ageing markers in COPD DOI: 10.1183/1393003.congress-2017.PA1018
  • Beclin1 circulating levels and accelerated aging markers in COPDPubmed:29402890
  • A novel combined score of biomarkers in sputum may be an indicator for lung cancer: A pilot studyDoi: 10.1016/j.cca.2018.09.027
  • Serum concentration of autophagy-related protein 7 is correlated with non-alcoholic fatty liver disease in Chinese adults
  • Crosstalk between vitamin D axis, inflammation and host immunity mechanisms: A prospective study33936265
  • Inhibition of HepG-2 Cells (Liver Cancer Cell Line) Viability by 3-Hydroxypyridine-2-Carboxaldehyde N (4)-Methylthiosemicarbazone
  • BIOMARKERS OF AUTOPHAGY AND ANTIVIRAL ROLE OF PLACENTA FOR PREDICTING PRETERM DELIVERY IN PATIENTS WITH PRETERM DELIVERY?¡­
  • IDENTIFICATION OF FACTORS CONNECTED WITH AUTOPHAGY ACTIVITY UNDER CONDITIONS OF PARTIAL NUTRITIONAL DEPRIVATION IN MEN

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