Polyclonal Antibody to Breast Cancer Susceptibility Protein 1 (BRCA1) Homo sapiens (Human) Polyclonal antibody

BRCAI; BRCC1; IRIS; PSCP; RNF53; Breast Cancer 1,Early Onset; RING finger protein 53

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB376Hu01-Recombinant Breast Cancer Susceptibility Protein 1 (BRCA1)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Breast Cancer Susceptibility Protein 1 (BRCA1) Packages (Simulation)
  • Polyclonal Antibody to Breast Cancer Susceptibility Protein 1 (BRCA1) Packages (Simulation)
  • Polyclonal Antibody to Breast Cancer Susceptibility Protein 1 (BRCA1) DAB staining on IHC-P; Samples: Human Liver cancer Tissue; Primary Ab: 30µg/ml Rabbit Anti-Human BR
  • Polyclonal Antibody to Breast Cancer Susceptibility Protein 1 (BRCA1) Figure. DAB staining on IHC-P; Samples: Human Prostate Gland Tissue.
  • Polyclonal Antibody to Breast Cancer Susceptibility Protein 1 (BRCA1) Figure. DAB staining on IHC-P; Samples: Human Prostate Gland Cancer Tissue.
  • Polyclonal Antibody to Breast Cancer Susceptibility Protein 1 (BRCA1) DAB staining on IHC-P;
    Samples: Human Kidney Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Human BRCA1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Breast Cancer Susceptibility Protein 1 (BRCA1) DAB staining on IHC-P;
    Samples: Human Prostate cancer Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Human BRCA1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAB376Hu01.jpg Figure. Western Blot; Sample: Recombinant BRCA1, Human.
  • Polyclonal Antibody to Breast Cancer Susceptibility Protein 1 (BRCA1) Figure. Western Blot; .Sample: Lane1: Human A431 Cells; Lane2: Human Hela Cells; Lane3: Mouse Testis Tissue.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against BRCA1. It has been selected for its ability to recognize BRCA1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 1-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat)

Citations

  • IN VITRO EFFECT OF RESVERATROL ON DNA METHYLATION AND BRCA1 EXPRESSION IN MCF-7 HUMAN BREAST CANCER CELLSPublication: 281344291

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